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BRD8 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB100-79780

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Predicted:

Mouse (100%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 25 and 75 of human Bromodomain containing 8 using the numbering given in TrEMBL entry Q969M9 (GeneID 10902).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for BRD8 Antibody - BSA Free

Western Blot: BRD8 Antibody [NB100-79780]

Western Blot: BRD8 Antibody [NB100-79780]

Western Blot: BRD8 Antibody [NB100-79780] - Detection of Human BRD8 by Western Blot. Samples: Nuclear extract (50 and 15 ug) from HeLa cells. Antibody: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation: BRD8 Antibody [NB100-79780]

Immunoprecipitation: BRD8 Antibody [NB100-79780]

Immunoprecipitation: BRD8 Antibody [NB100-79780] - Detection of human BRD8 by western blot of immunoprecipitates. Samples: Nuclear extract (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-BRD8 antibody NB100-79780 used for IP at 6 ug per reaction. BRD8 was also immunoprecipitated by rabbit anti-BRD8 antibody NB100-79781. For blotting immunoprecipitated BRD8, NB100-79780 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for BRD8 Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000 - 1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: BRD8

The protein encoded by this gene interacts with thyroid hormone receptor in a ligand-dependent manner and enhances thyroid hormone-dependent activation from thyroid response elements. This protein contains a bromodomain and is thought to be a nuclear receptor coactivator. Three alternatively spliced transcript variants that encode distinct isoforms have been identified.

Long Name

Bromodomain-containing protein 8

Alternate Names

SMAP, TrCP120

Entrez Gene IDs

10902 (Human)

Gene Symbol

BRD8

UniProt

Additional BRD8 Products

Product Documents for BRD8 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BRD8 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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