BPHL Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-00178
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
ELISA, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with purified, recombinant Human BPHL (Uniprot#: Q86WA6-1; Ser38-Gln291).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2C to 8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Scientific Data Images for BPHL Antibody - Azide and BSA Free
Immunoprecipitation: BPHL Antibody [NBP3-00178] -
Immunoprecipitation: BPHL Antibody [NBP3-00178] - BPHL was immunoprecipitated using: Lane A:0.5 mg 293T Whole Cell Lysate 0.5 uL NBP3-00178 and 15 ul of 50 % Protein G agarose. Primary antibody: NBP3-00178, at 1:500 dilution. Secondary antibody: Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution. Developed using the odssey technique. Performed under reducing conditions. Predicted band size: 31 kDa Observed band size: 31 kDa
Western Blot: BPHL Antibody [NBP3-00178] -
Western Blot: BPHL Antibody [NBP3-00178] - NBP3-00178 at 1:500 dilution Lane A: THP1 Whole Cell Lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:32 kDa Observed band size:30 kDa (We are unsure as to the identity of these extra bands.)
Immunohistochemistry-Paraffin: BPHL Antibody [NBP3-00178] -
Immunohistochemistry-Paraffin: BPHL Antibody [NBP3-00178] - Immunochemical staining of human BPHL in human kidney with NBP3-00178 (1:10000, formalin-fixed paraffin embedded sections).Applications for BPHL Antibody - Azide and BSA Free
Application
Recommended Usage
ELISA
1:25000-1:50000
Immunohistochemistry-Paraffin
1:5000-1:20000
Immunoprecipitation
0.2-1 uL/mg of lysate
Western Blot
1:500-1:1000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
0.2 um filtered solution in PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BPHL
Alternate Names
biphenyl hydrolase-like (serine hydrolase), Biphenyl hydrolase-like protein, Biphenyl hydrolase-related protein, Bph-rp, Breast epithelial mucin-associated antigen, EC 3.1, MCNAA, MGC125930, MGC41865, VACVASE, valacyclovir hydrolase, valacyclovirase
Gene Symbol
BPHL
Additional BPHL Products
Product Documents for BPHL Antibody - Azide and BSA Free
Product Specific Notices for BPHL Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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