BPDE-DNA Antibody (5D11)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11198

Discontinued Product
NBP3-11198 has been discontinued. View all BPDE-DNA products.

Key Product Details

Species Reactivity

Non-species specific

Applications

Flow Cytometry, Immunoassay, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5D11

Concentration

0.1 mg/ml

View all BPDE-DNA Antibodies »

Product Specifications

Immunogen

BPDE-I-DNA complexed with methylated BSA

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for BPDE-DNA Antibody (5D11)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunoassay

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
For immunohistochemistry dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:10.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

0.2 um filtered solution in PBS, 0.1% BSA

Preservative

0.02% Sodium Azide

Concentration

0.1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: BPDE-DNA

A number of chemicals, including polycyclic aromatic hydrocarbons (PAHs), have been shown to bind to DNA. This DNA damage can occur both early and late in the malignant process, thereby acting as an initiator and assisting in the progression of tumors. PAHs are released into the environment following incomplete combustion of organic materials. The most common sources of PAHs are from smoking and from consuming broiled or grilled foods. Human exposure to PAHs comes from various occupational, environmental, dietary and medicinal sources. Benzo[a]pyrene is a representative PAH. Antibodies to benzo[a]pyrenediol-epoxide modified DNA (BPDE-DNA) can be used to identify polycyclic aromatic hydrocarbon (PAH)-DNA adducts. Exposure to this group of compounds is believed to be carcinogenic. The monoclonal antibody 5D11 recognizes BPDE-I-DNA (PAH-DNA).

Alternate Names

Benzo Pyrene Dihydrodiol Epoxide, Benzo(a)pyrene, Benzo(a)pyrenediol-epoxide, Benzo(a)pyrenediol-epoxide modified DNA, BPDE-DNA adduct, BPDE-I-DNA, PAH-DNA adduct, Polycyclic aromatic hydrocarbon-DNA adduct

Product Documents for BPDE-DNA Antibody (5D11)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for BPDE-DNA Antibody (5D11)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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