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Key Product Details

Species Reactivity

Human, Mouse, Rat, Bacteria, Monkey

Applications

Dot Blot, ELISA, Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry Free-Floating, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # MOAB-2

Format

BSA Free

Concentration

1.0 mg/ml

Product Summary for beta Amyloid Antibody (MOAB-2) - BSA Free

Immunogen

This beta Amyloid antibody was developed against recombinant human beta Amyloid 42.

Epitope

Residues 1-4 of beta Amyloid

Reactivity Notes

Use in Mouse reported in scientific literature (PMID:35401818). Monkey reactivity reported in scientific literature (PMID: 29241829). Use in Bacteria reported in scientific literature (PMID:32413239).

Specificity

Beta Amyloid antibody (MOAB-2) recognizes unaggregated, oligomeric and fibrillar forms of beta Amyloid 42 and unaggregated beta Amyloid 40. Does not detect APP.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for beta Amyloid Antibody (MOAB-2) - BSA Free

Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

Immunohistochemical Staining of beta Amyloid in Normal and 5xFAD Mouse Brain

IHC analysis of beta Amyloid on normal mouse brain (left) and 5xFAD mouse brain (right) using DAB with hematoxylin counterstain. The MOAB-2 antibody was used at 1:20 on normal mouse brain and at 1:400 on 5xFAD mouse brain.
Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Western Blot Detection of beta Amyloid in Mouse Brain Homogenate

Analysis of beta Amyloid (MOAB-2) antibody in (1) 100 pmole beta Amyloid 42, (2) 5xFAD mouse brain homogenate Repetition 1 and (3) 5xFAD mouse brain homogenate Repetition 2.
Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western Blot Analysis of HEK-APP SWE/BACE1 Cell Lysates Using beta Amyloid Antibody

Western blot analysis in cell lysates from HEK-APP SWE/BACE1 cells probed with an antibody against the C-terminus of APP (Lanes 1 and 2) and beta Amyloid (MOAB-2, Lanes 3 and 4). Beta Amyloid (MOAB-2) does not detect APP (from PMID: 22423893).

Applications for beta Amyloid Antibody (MOAB-2) - BSA Free

Application
Recommended Usage

Dot Blot

reported in scientific literature (PMID 22423893)

ELISA

1:100-1:1000

Immunoblotting

reported in scientific literature (PMID 28314768)

Immunocytochemistry/ Immunofluorescence

1:200-1:500

Immunohistochemistry

1:200-1:1000

Immunohistochemistry Free-Floating

reported in scientific literature (PMID 25747037)

Immunohistochemistry-Paraffin

1:200-1:1000

Immunoprecipitation

1:200-1:1000

Western Blot

1:1000-1:5000
Application Notes
In Western blot, a band can be seen at ~4 kDa, representing the beta Amyloid monomer. Larger bands may also be seen representing the unaggregated, oligomeric, and fibrillar forms of beta Amyloid. For higher beta Amyloid yield in WB, please follow the extraction protocol described in Youmans et al, J Neurosci Methods. 2011 March 15; 196(1): 51-59 (PMID: 21219931).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 2 reviews rated 4.5 using NBP2-13075 in the following applications:

Published Applications

Read 66 publications using NBP2-13075 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Amyloid beta

Beta amyloid, also known as Abeta, is a peptide that varies in length from 39 to 43 amino acids and is processed from the transmembrane protein amyloid precursor protein (APP) by beta-secretase (BACE-1) and gamma-secretase. Beta amyloid [1-40], beta amyloid [1-42], and beta amyloid [1-43] peptides result from cleavage of APP after residues 40, 42, and 43, respectively. The aggregation of beta amyloid monomers produces insoluble oligomers and protofibrils. The longer "stickier" forms of beta amyloid, particularly ABeta42, are known as the main component of amyloid plaques, which are deposits (senile plaques or neuritic plaques) found in the brains of patients with Alzheimer's disease (AD) (1,2). Research indicates that intraneuronal beta amyloid accumulation may be an important proximal neurotoxic event in the pathogenesis of Alzheimer's disease. Abeta deposits have also been reported in the hearts of AD patients (3).

Pyroglutamate amyloid beta peptides (pGlu-Abeta) are N-terminal truncations in which the N-terminal glutamate is cyclized to pyroglutamate resulting in pGlu-Abeta (3-40/42 and 11-40/42) (4). This pyrE modification may have a greater propensity to aggregate under physiological conditions and has been implicated as the molecular species responsible for seeding larger oligomers of amyloid beta.

References

1. Chen GF, Xu TH, Yan Y, Zhou YR, Jiang Y, Melcher K, Xu HE. (2017) Amyloid beta: structure, biology and structure-based therapeutic development. Acta Pharmacol Sin. 38(9):1205-1235. PMID: 28713158

2. De-Paula VJ1, Radanovic M, Diniz BS, Forlenza OV. (2012) Alzheimer's disease. Subcell Biochem. 65:329-52. PMID: 23225010

3. Schaich CL, Maurer MS, Nadkarni NK. (2019) Amyloidosis of the Brain and Heart: Two Sides of the Same Coin? JACC Heart Fail. 7(2):129-131. PMID: 30704604

4. He W, Barrow CJ. (1999) The A beta 3-pyroglutamyl and 11-pyroglutamyl peptides found in senile plaque have greater beta-sheet forming and aggregation propensities in vitro than full-length A beta. Biochemistry. 38(33):10871-7. PMID: 10451383

Alternate Names

Abeta, beta-Amyloid Peptide

Entrez Gene IDs

351 (Human)

Gene Symbol

APP

UniProt

Product Documents for beta Amyloid Antibody (MOAB-2) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for beta Amyloid Antibody (MOAB-2) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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FAQs for beta Amyloid Antibody (MOAB-2) - BSA Free

Showing  1 - 2 of 2 FAQs Showing All
  • Q: Does your antibody beta Amyloid Antibody (MOAB-2) # NBP2-13075 work on frozen sections for histology?

    A: This antibody has never been tested on frozen sections. It has been validated in ICC, IP and IHC on paraffin-embedded tissues, so it may work on frozen sections. While we cannot guarantee that it will work on frozen sections, if you would like to test NBP2-13075 in an untested application and share your results with us, I can recommend our Innovator's Reward Program. Our Innovator's Reward program was created to allow researchers the opportunity to try our primary antibodies in an untested species or application, without the financial risk of failure. To participate you simply submit an online review detailing your positive or negative results. In return, you receive a discount voucher for 100% of the purchase price of the reviewed product. Reviews may also be submitted by emailing innovators@novusbio.com.

  • Q: Please provide the epitope region information for NBP2-13075 Beta Amyloid Antibody.

    A: The epitope is a.a. 672 - 675 and please use https://www.uniprot.org/uniprot/P05067#sequences to check all important information.

Showing  1 - 2 of 2 FAQs Showing All
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