Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80592
Key Product Details
Species Reactivity
Human
Applications
Western Blot, Flow Cytometry, Flow (Intracellular), Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgM Clone # 20F248
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to the N-terminal portion of the human Bax inhibitor 1 protein (between amino acids 1-40) UniProt P55061.
Epitope
Epitope mapping indicates that the antibody recognizes an epitope in the N-terminal region of Bax inhibitor 1.
Clonality
Monoclonal
Host
Mouse
Isotype
IgM
Description
Novus Biologicals Mouse Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free (NBP2-24949) is a monoclonal antibody validated for use in WB, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free
Western Blot: Bax inhibitor 1 Antibody (20F248)Azide and BSA Free [NBP2-80592]
Western Blot: Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free [NBP2-80592] - Analysis of Bi-1 in A) A549 and B) human lung lysate using this antibody at 1:1000. Image from the standard format of this antibody.Immunocytochemistry/ Immunofluorescence: Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free [NBP2-80592]
Immunocytochemistry/Immunofluorescence: Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free [NBP2-80592] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti Bax inhibitor 1 (20F248), NBP2-24949, at a 1:200 dilution overnight at 4C and detected with an anti-mouse IgM Dylight 488 (Green) at a 1:500 dilution. Actin was detected with Phalloidin 568 (Red) at a 1:200 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective. Image from the standard format of this antibody.Applications for Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
1 ug/ml
Immunocytochemistry/ Immunofluorescence
1:200
Western Blot
1:500-1:1000
Application Notes
This antibody is Cytof ready.
Formulation, Preparation, and Storage
Purification
IgM purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Bax inhibitor 1
Alternate Names
BAXI1, BI1, BI-1Transmembrane BAX inhibitor motif-containing protein 6, TEGTbax inhibitor 1, testis enhanced gene transcript, Testis-enhanced gene transcript protein, transmembrane BAX inhibitor motif containing 6
Gene Symbol
TMBIM6
Additional Bax inhibitor 1 Products
Product Documents for Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free
Product Specific Notices for Bax inhibitor 1 Antibody (20F248) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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