Basal cell cytokeratin Antibody (RCK103) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97729

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-97729
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Human, Rat, Porcine, Avian, Canine, Chicken, Guinea Pig, Hamster, Rabbit, Zebrafish

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # RCK103

Format

BSA Free

Concentration

1 mg/ml

View all Basal cell cytokeratin Antibodies »

Product Specifications

Immunogen

Derived by fusion of SP2/0-Ag14 Mouse myeloma cells with spleen cells from a BALB/c Mouse immunized with a mix of cell preparations containing Human Cytokeratins.

Specificity

This antibody is a Cytokeratin antibody recognizing (amongst others) Cytokeratin 5. This monoclonal antibody stains basal cells in combined and stratified epithelial tissues. It recognizes the stem cell population, including the so-called amplifying cells in the prostate epithelium.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

The antibody is shipped at ambient temperature and may be stored at 4C. For prolonged storage prepare appropriate aliquots and store at or below -20C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

Scientific Data Images for Basal cell cytokeratin Antibody (RCK103) - BSA Free

Immunocytochemistry/ Immunofluorescence: Basal cell cytokeratin Antibody (RCK103) - BSA Free [NBP1-97729]

Immunocytochemistry/ Immunofluorescence: Basal cell cytokeratin Antibody (RCK103) - BSA Free [NBP1-97729]

Immunocytochemistry/Immunofluorescence: Basal cell cytokeratin Antibody (RCK103) [NBP1-97729] - Staining of a 7 days old zebrafish embryo, showing positive reactivity in epithelia lining the intestine and internal organs
Immunohistochemistry-Frozen: Basal cell cytokeratin Antibody (RCK103) - BSA Free [NBP1-97729]

Immunohistochemistry-Frozen: Basal cell cytokeratin Antibody (RCK103) - BSA Free [NBP1-97729]

Immunohistochemistry-Frozen: Basal cell cytokeratin Antibody (RCK103) [NBP1-97729] - Frozen sections of human skin

Applications for Basal cell cytokeratin Antibody (RCK103) - BSA Free

Application
Recommended Usage

Flow Cytometry

1:100-1:200

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:100-1:200

Western Blot

1:100-1:1000
Application Notes
Optimal antibody dilution should be determined by titration; recommended range is 1:100 - 1:200 for flow cytometry, and for immunohistochemistry with avidin-biotinylated Horseradish peroxidase complex (ABC) as detection reagent, and 1:100 - 1:1000 for immunoblotting applications.

This antibody is CyTOF ready..

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS, pH 7.2

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Basal cell cytokeratin

Additional Basal cell cytokeratin Products

Product Documents for Basal cell cytokeratin Antibody (RCK103) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Basal cell cytokeratin Antibody (RCK103) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.