BARHL1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86513
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Human, Mouse, Rat
Predicted:
Rat (96%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunocytochemistry/ Immunofluorescence, IF/IHC, Protein Array
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: LELSPRSESSSDCSSPASPGRDCLETGTPRPGGASGPGLDSHLQPGQLSAPAQSRTVTSSFLIRDILADCKPLAACAPYSSSGQPAAPEPGGRLAAKAAEDFRDKLDKSGSNASSDSEYKVKEEGDREISSSRDSP
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for BARHL1 Antibody - BSA Free
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of human tonsil shows no positivity in non-germinal center cells as expected.Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of mouse embryo E14 shows strong nuclear positivity in neuronal cells in developing brain stem and cerebellum.Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513]
Immunohistochemistry-Paraffin: BARHL1 Antibody [NBP1-86513] - Staining of human cerebellum shows weak nuclear positivity in cells in molecular layer.Applications for BARHL1 Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1-10µg per reaction
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended. Please note that ICC/IF was reported in PMID 31641054, which used a previous lot that is no longer available. The current lot of this antibody is not validated for use in ICC/IF. Please contact technical services if you have any questions.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: BARHL1
Alternate Names
BarH (Drosophila)-like 1, barH-like 1 homeobox protein, BarH-like homeobox 1
Gene Symbol
BARHL1
Additional BARHL1 Products
Product Documents for BARHL1 Antibody - BSA Free
Product Specific Notices for BARHL1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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