ATG12 Antibody (EPR4800)

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96569

Discontinued Product
NBP1-96569 has been discontinued. View all ATG12 products.

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Rabbit Clone # EPR4800

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all ATG12 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to residues on the C-terminus in human Atg12 was used as an immunogen.

Clonality

Monoclonal

Host

Rabbit

Applications for ATG12 Antibody (EPR4800)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:250-500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:250-500

Western Blot

1:1000-10000

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

49% PBS, 0.05% BSA and 50% Glycerol

Preservative

0.01% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ATG12

Autophagy is a process of bulk protein degradation in which cytoplasmic components, including organelles, are enclosed in double-membrane structures called autophagosomes and delivered to lysosomes or vacuoles for degradation. Atg12 is the human homolog of a yeast protein involved in autophagy (1). It is essential for Atg12 conjugation, and the Atg12-Atg5 conjugate is necessary to form autophagic vesicles (2). The E3-like activity of the Atg12-Atg5 conjugate potentiates the formation of Atg8-PE; making the autophagic pathway analogous to ubiquitin-like pathways where an E3 conjugation ligase is required (3).

Long Name

ATG12 Autophagy Related 12 Homolog

Alternate Names

APG12, FBR93, HAPG12

Entrez Gene IDs

9140 (Human); 67526 (Mouse); 361321 (Rat)

Gene Symbol

ATG12

UniProt

O94817

Additional ATG12 Products

Product Documents for ATG12 Antibody (EPR4800)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ATG12 Antibody (EPR4800)

Produced using Abcam's RabMab&#174 technology. RabMab&#174 technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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