Asporin Antibody

Western Blot: Asporin Antibody [NB100-1514]

Western Blot: Asporin Antibody [NB100-1514] - (0.1ug/ml) staining of Human Tonsil (A), (0.3ug/ml) Human Uterus (B), Mouse Skeletal Muscle (C) (1ug/ml) Rat Skeletal Muscle (D) and negative control Human Cerebellum (E) lysate (35ug protein in RIPA buffer).Detected by chemiluminescence.

Immunocytochemistry/ Immunofluorescence: Asporin Antibody [NB100-1514]

Immunocytochemistry/Immunofluorescence: Asporin Antibody [NB100-1514] - analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml), showing nuclear membrane staining. The nuclear stain is DA

Western Blot: Asporin Antibody [NB100-1514] -

SOX9 and ASPN promote both CF-to-MF and CF-to-OF transition.(A) AngII- and TGF-beta –induced expression of Sox9 and Aspn transcripts in CF upon Sox9 or Aspn knockdown as assessed by qPCR. (B) SOX9 and ASPN protein expression in CF upon Sox9 or Aspn knockdown with representative Western blots. (C) AngII- and TGF-beta –induced CF proliferation upon Sox9 or Aspn knockdown as assessed by CCK8 assay. (D) CF migration upon Sox9 or Aspn knockdown as assessed by scratch wound assay. (E) AngII- and TGF-beta –induced CF collagen production upon Sox9 or Aspn knockdown as assessed by soluble Sirius red assay. (F) AngII- and TGF-beta –induced alphaSMA expression in CF upon Sox9 or Aspn knockdown as assessed by immunofluorescence. Scale bar: 10 μm. (G) Osteogenic growth medium–induced calcium nodules (dark red, arrows) in CF upon Sox9 or Aspn knockdown as assessed by alizarin red staining. Scale bar: 20 μm. (H and I) AngII- and TGF-beta –induced expression of Postn, Runx2, and Alp3 transcripts in CF upon Sox9 or Aspn knockdown as assessed by qPCR. (J) Expression of active beta-catenin in CF upon Sox9 or Aspn knockdown as assessed by Western blot. Data presented as mean +/- SEM; n = 3–4 independent experiments. Student’s t test (B) or ANOVA with Holm-Bonferroni post hoc correction. #P < 0.05 versus NC mimic ctrl stimulus, ##P < 0.01 versus NC mimic ctrl stimulus, ###P < 0.001 versus NC mimic ctrl; *P < 0.05, **P < 0.01, ***P < 0.001. AngII, Angiotensin II; NC, negative control. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37154157), licensed under a CC-BY license. Not internally tested by Novus Biologicals.