Aspartate Transaminase Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77739
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Porcine
Applications
Western Blot, ELISA
Label
Biotin
Antibody Source
Polyclonal Sheep IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Aspartate Transaminase Antibody was produced by repeated immunizations with Aspartate Transaminase from pig heart. (Uniprot: P00503)
Reactivity Notes
Cross reactivity against Aspartate Transaminase (AST) from other tissues and species may occur but have not been specifically determined
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]
This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Aspartate Aminotransferase (AST) [Pig Heart]
Applications for Aspartate Transaminase Antibody [Biotin]
Application
Recommended Usage
ELISA
1:5000 - 1:25000
Western Blot
1:500 - 1:3000
Application Notes
This product has been assayed against 1.0 ug of Aspartate Aminotransferase (AST in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 100 ul deionized water (or equivalent)
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: Aspartate Transaminase
Alternate Names
aspartate aminotransferase, cytoplasmic, CASPAT
Gene Symbol
GOT1
UniProt
Additional Aspartate Transaminase Products
Product Documents for Aspartate Transaminase Antibody [Biotin]
Product Specific Notices for Aspartate Transaminase Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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