ASAP1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77934
Key Product Details
Species Reactivity
Validated:
Mouse
Predicted:
Bovine (92%), Human (92%), Rat (96%). Backed by our 100% Guarantee.
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This affinity purified ASAP1 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 775-800 of mouse ASAP1 protein. (Uniprot: Q9QWY8)
Reactivity Notes
A BLAST analysis was used to suggest cross reactivity with ASAP1 proteins from human, chicken, bovine, dog, rat and chimpanzee based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known
Specificity
This affinity-purified antibody is directed against mouse ASAP1 protein. Reactivity occurs against both the phosphorylated and non-phosphorylated forms of the protein at residue Y782.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This affinity-purified antibody is directed against mouse ASAP1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
Scientific Data Images for ASAP1 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: ASAP1 Antibody [NBP1-77934]
Immunocytochemistry/Immunofluorescence: ASAP1 Antibody [NBP1-77934] - Analysis of ASAP1present in mouse NIH3T3 cells transfected with activated Src. Specific staining is not present when antibody is pre-incubated with the immunizing peptide prior to reaction with cells.Applications for ASAP1 Antibody - BSA Free
Application
Recommended Usage
ELISA
1:2000-1:10000
Immunocytochemistry/ Immunofluorescence
1:10-1:500
Immunohistochemistry
1:20 - 1:50
Immunohistochemistry-Paraffin
1:1000-1:2500
Western Blot
1:500-1:2000
Application Notes
This affinity purified antibody has been tested for use in ELISA, IF microscopy and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 130 kDa in size corresponding to ASAP1 protein by western blotting in the appropriate cell lysate or extract. This antibody recognizes both phosphorylated and non-phosphorylated ASAP1 at amino acid Y782.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Format
BSA Free
Preservative
0.01% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.
Background: ASAP1
Alternate Names
AMAP1, CENTB4, DDEF1, PAG2, ZG14P
Gene Symbol
ASAP1
UniProt
Additional ASAP1 Products
Product Documents for ASAP1 Antibody - BSA Free
Product Specific Notices for ASAP1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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