ASAP1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19159

Novus Biologicals, part of Bio-Techne
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NBP1-19159
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all ASAP1 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 750 and 800 of human ArfGAP with SH3 domain, ankyrin repeat and PH domain 1 using the numbering given in entry NP_060952.2 (GeneID 50807).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ASAP1 Antibody

Western Blot: ASAP1 Antibody [NBP1-19159]

Western Blot: ASAP1 Antibody [NBP1-19159]

Western Blot: ASAP1 Antibody [NBP1-19159] - ASAP1 by Western Blot and Immunoprecipitation: [NBP1-19159]- Whole cell lysate (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells used for WB at 0.04 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. ASAP1 was also immunoprecipitated by rabbit anti-ASAP1 antibody NBP1-19158, which recognizes an upstream epitope.

Applications for ASAP1 Antibody

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: ASAP1

ASAP1 ( Arf-Gap with SH3 domain, ANK repeat and PH domain-containing protein 1) is an Arf (ADP-ribosylation factor) GAP (GTPase-activating protein) that catalyzes the hydrolysis of GTP bound to Arf, a small GTP-binding protein. As a regulator of Arf activity ASAP1 has been shown to regulate cell adhesion and migration, and to be involved in carcinogenesis. Structurally, ASAP1 bears two ankyrin repeats, one Arf-GAP domain, one PH domain, one SH3 domain, and an N-terminal Bin, Amphiphysin and Rvs167 (BAR) domain. Alternative names for ASAP1 include PIP2-dependent ARF1 GAP, ARF GTPase-activating protein 1, development and differentiation-enhancing factor 1, differentiation-enhancing factor 1, PAP, PAG2, AMAP1, ZG14P, CENTB4, DEF-1, DDEF1, and KIAA1249.

Alternate Names

AMAP1, CENTB4, DDEF1, PAG2, ZG14P

Entrez Gene IDs

50807 (Human)

Gene Symbol

ASAP1

UniProt

NP_060952.2 (Human)

Additional ASAP1 Products

Product Documents for ASAP1 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for ASAP1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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