Arginyl tRNA synthetase Antibody (4Y1H4)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-33518
Key Product Details
Species Reactivity
Human, Mouse
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Rabbit IgG Clone # 4Y1H4
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human Arginyl tRNA synthetase (NP_002878.2).
Sequence:
MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVGHLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGK
Sequence:
MDVLVSECSARLLQQEEEIKSLTAEIDRLKNCGCLGASPNLEQLQEENLKLKYRLNILRKSLQAERNKPTKNMINIISRLQEVFGHAIKAAYPDLENPPLLVTPSQQAKFGDYQCNSAMGISQMLKTKEQKVNPREIAENITKHLPDNECIEKVEIAGPGFINVHLRKDFVSEQLTSLLVNGVQLPALGENKKVIVDFSSPNIAKEMHVGHLRSTIIGESISRLFEFAGYDVLRLNHVGDWGTQFGMLIAHLQDKFPDYLTVSPPIGDLQVFYKESKKRFDTEEEFKKRAYQCVVLLQGK
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
75 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Arginyl tRNA synthetase Antibody (4Y1H4)
Immunocytochemistry/ Immunofluorescence: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] -
Immunocytochemistry/ Immunofluorescence: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] - Confocal imaging of paraffin-embedded Mouse brain tissue using Arginyl tRNA synthetase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.
Immunocytochemistry/ Immunofluorescence: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] -
Immunocytochemistry/ Immunofluorescence: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] - Confocal imaging of paraffin-embedded Mouse brain tissue using Arginyl tRNA synthetase Rabbit mAb followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer(pH 6.0) prior to IF staining. Objective: 40x.
Western Blot: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] -
Western Blot: Arginyl tRNA synthetase Antibody (4Y1H4) [NBP3-33518] - Western Blot analysis of lysates from 293T cells using Arginyl tRNA synthetase Rabbit mAb at 1:1000 dilution incubated overnight at 4C.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for Arginyl tRNA synthetase Antibody (4Y1H4)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 ug/mL
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.05% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Arginyl tRNA synthetase
Alternate Names
arginine tRNA ligase 1, cytoplasmic, Arginine--tRNA ligase, arginyl-tRNA synthetase, arginyl-tRNA synthetase, cytoplasmic, ArgRS, DALRD1, EC 6.1.1.19, MGC8641
Gene Symbol
RARS1
Additional Arginyl tRNA synthetase Products
Product Documents for Arginyl tRNA synthetase Antibody (4Y1H4)
Product Specific Notices for Arginyl tRNA synthetase Antibody (4Y1H4)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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