AP-2 beta/TFAP2B Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89063
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Rat (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: QRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMG
Reactivity Notes
Mouse reactivity reported in scientific literature (PMID: 28894266).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for AP-2 beta/TFAP2B Antibody - BSA Free
Immunohistochemistry-Paraffin: AP-2 beta/TFAP2B Antibody [NBP1-89063]
Immunohistochemistry-Paraffin: AP-2 beta/TFAP2B Antibody [NBP1-89063] - Staining of human epididymis shows strong nuclear positivity in glandular cells.Chromatin Immunoprecipitation-exo-Seq: AP-2 beta/TFAP2B Antibody - BSA Free [NBP1-89063]
ChIP-Exo-Seq composite graph for Anti-TFAP2B (NBP1-89063) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.Western Blot: AP-2 beta/TFAP2B Antibody - BSA Free [NBP1-89063] -
Copy number of the AK029562 gene as well as AP-2 beta/AP-2 delta mRNA and protein levels in the eyes of WT and transgenic animals. (A) The panel illustrates the copy number of the AK029562 gene in WT, non-glaucomatous Mut-XDHTg and glaucomatous Mut-XDHTg mice. **Significantly different (p < 0.01) after Student’s t-test. N = the number of animals analyzed. Each value is expressed as the Mean +/- SD. (B) The expression levels of the AP-2 beta and AP-2 delta mRNAs in WT, non-glaucomatous Mut-XDHTg as well as glaucomatous Mut-XDHTg animals are indicated. Each value is the mean +/- SD of 7 distinct animals. **Significantly different (p < 0.01) after Student’s t-test. (C) The amounts of AP-2 beta and AP-2 delta proteins in the eyes of WT, non-glaucomatous Mut-XDHTg and glaucomatous Mut-XDHTg animals were measured by Western blot analysis. Each lane represents a single animal. The upper bar graphs illustrate the results obtained after densitometric analysis of the AP-2 beta and AP-2 delta bands and normalization for the tubulin band. **Significantly different (p < 0.01) after Student’s t-test. The full-length blots/gels are presented in Supplementary Figure S2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28894266), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for AP-2 beta/TFAP2B Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation-exo-Seq
1-10ug per reaction
Immunohistochemistry
1:200 - 1:500
Immunohistochemistry-Paraffin
1:200 - 1:500
Western Blot
Reported in scientific literature (PMID: 28894266)
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: AP-2 beta
Long Name
Activating Enhancer-binding Protein 2 beta
Alternate Names
AP2 beta, CHAR, TFAP2B
Gene Symbol
TFAP2B
Additional AP-2 beta Products
Product Documents for AP-2 beta/TFAP2B Antibody - BSA Free
Product Specific Notices for AP-2 beta/TFAP2B Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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