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AP-2 beta/TFAP2B Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-89063

Novus Biologicals, part of Bio-Techne
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NBP1-89063
NBP1-89063-25ul

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Mouse

Predicted:

Rat (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Chromatin Immunoprecipitation-exo-Seq

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: QRQRQEVGSEAGSLLPQPRAALPQLSGLDPRRDYHSVRRPDVLLHSAHHGLDAGMG

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 28894266).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for AP-2 beta/TFAP2B Antibody - BSA Free

Immunohistochemistry-Paraffin: AP-2 beta/TFAP2B Antibody [NBP1-89063]

Immunohistochemistry-Paraffin: AP-2 beta/TFAP2B Antibody [NBP1-89063]

Immunohistochemistry-Paraffin: AP-2 beta/TFAP2B Antibody [NBP1-89063] - Staining of human epididymis shows strong nuclear positivity in glandular cells.
AP-2 beta/TFAP2B Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: AP-2 beta/TFAP2B Antibody - BSA Free [NBP1-89063]

Chromatin Immunoprecipitation-exo-Seq: AP-2 beta/TFAP2B Antibody - BSA Free [NBP1-89063]

ChIP-Exo-Seq composite graph for Anti-TFAP2B (NBP1-89063) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
AP-2 beta/TFAP2B Antibody - BSA Free

Western Blot: AP-2 beta/TFAP2B Antibody - BSA Free [NBP1-89063] -

Copy number of the AK029562 gene as well as AP-2 beta/AP-2 delta mRNA and protein levels in the eyes of WT and transgenic animals. (A) The panel illustrates the copy number of the AK029562 gene in WT, non-glaucomatous Mut-XDHTg and glaucomatous Mut-XDHTg mice. **Significantly different (p < 0.01) after Student’s t-test. N = the number of animals analyzed. Each value is expressed as the Mean +/- SD. (B) The expression levels of the AP-2 beta and AP-2 delta mRNAs in WT, non-glaucomatous Mut-XDHTg as well as glaucomatous Mut-XDHTg animals are indicated. Each value is the mean +/- SD of 7 distinct animals. **Significantly different (p < 0.01) after Student’s t-test. (C) The amounts of AP-2 beta and AP-2 delta proteins in the eyes of WT, non-glaucomatous Mut-XDHTg and glaucomatous Mut-XDHTg animals were measured by Western blot analysis. Each lane represents a single animal. The upper bar graphs illustrate the results obtained after densitometric analysis of the AP-2 beta and AP-2 delta bands and normalization for the tubulin band. **Significantly different (p < 0.01) after Student’s t-test. The full-length blots/gels are presented in Supplementary Figure S2. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28894266), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for AP-2 beta/TFAP2B Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

Reported in scientific literature (PMID: 28894266)
Application Notes
IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: AP-2 beta

The TFAP2B gene encodes a member of the AP-2 family of transcription factors. AP-2 proteins form homo- or hetero-dimers with other AP-2 family members and bind specific DNA sequences. They are thought to stimulate cell proliferation and suppress terminal differ

Long Name

Activating Enhancer-binding Protein 2 beta

Alternate Names

AP2 beta, CHAR, TFAP2B

Gene Symbol

TFAP2B

Additional AP-2 beta Products

Product Documents for AP-2 beta/TFAP2B Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for AP-2 beta/TFAP2B Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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