ANKRD27 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-50030
![Western Blot: ANKRD27 Antibody [NBP1-50030]](https://resources.bio-techne.com/images/products/ANKRD27-Antibody-Western-Blot-NBP1-50030-img0003.jpg "Western Blot: ANKRD27 Antibody [NBP1-50030]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies, Biological Validation
Species Reactivity
Validated:
Human
Predicted:
Primate (100%). Backed by our 100% Guarantee.
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 1000 and 1050 of human Vps9 Domain and Ankyrin-Repeat-Containing Protein using the numbering given in entry NP_115515.2 (GeneID 84079).
Reactivity Notes
Orangutan (100%).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit ANKRD27 Antibody - BSA Free (NBP1-50030) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for ANKRD27 Antibody - BSA Free
Western Blot: ANKRD27 Antibody [NBP1-50030]
Western Blot: ANKRD27 Antibody [NBP1-50030] - Detection of Human Varp by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. Antibodies: Affinity purified rabbit anti-Varp antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 6 mcg/mg lysate. Varp was also immunoprecipitated by rabbit anti-Varp antibody which recognizes an upstream epitope. For blotting immunoprecipitated Varp, the ReliaBLOT Reagents and Procedures were used. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).Applications for ANKRD27 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: ANKRD27
Alternate Names
ankyrin repeat domain 27 (VPS9 domain), ankyrin repeat domain-containing protein 27, DKFZp434L0718, FLJ00040, VARP, Vps9 domain and ankyrin-repeat-containing protein, VPS9 domain-containing protein, VPS9-ankyrin-repeat protein
Gene Symbol
ANKRD27
Additional ANKRD27 Products
Product Documents for ANKRD27 Antibody - BSA Free
Product Specific Notices for ANKRD27 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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