Alkaline Phosphatase, Intestinal Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB120-7325

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NB120-7325-100ug
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Bovine

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

LYOPH mg/ml

View all Alkaline Phosphatase, Intestinal Antibodies »

Product Specifications

Immunogen

Alkaline Phosphatase, Intestinal, Intestinal [Calf Intestine] (Uniprot: P19111)

Reactivity Notes

Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.

Localization

Attached to the membrane by a GPI-anchor.

Specificity

Cross reactivity against Alkaline Phosphatase, Intestinal from other tissues and species may occur but have not been specifically determined.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

This antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]

Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Alkaline Phosphatase, Intestinal Antibody - BSA Free

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Western Blot of Rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane.Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT.Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT.Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT.Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase." Image using the Biotin form of this antibody.
Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Alkaline Phosphatase (Calf Intestine). Load: 50 ng per lane. Primary antibody: Rabbit anti-Alkaline Phosphatase (Calf Intestine) Antibody at 1:1,000 overnight at 4C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 57 kDa, 60 kDa for Alkaline Phosphatase (Calf Intestine).
Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325]

Western Blot: Alkaline Phosphatase, Intestinal Antibody [NB120-7325] - Lane 1: Calf intestine Alkaline Phosphatase. Load: 1.5 ug Block: 5% Blotto 1 hour at 4C. Primary antibody: diluted to 1:1000 in 5% Blotto overnight at 4C. Secondary antibody: Goat anti Rabbit 1:20,000 in 1 Hour 4C Substrate: Femtomax 110.

Applications for Alkaline Phosphatase, Intestinal Antibody - BSA Free

Application
Recommended Usage

ELISA

1:20000-1:100000

Immunohistochemistry

1:1000-1:5000

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:2000-1:10000
Application Notes
This product has been tested against 1.0 ug of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Formulation, Preparation, and Storage

Purification

Multi-step

Reconstitution

Reconstitute with 100 ul deionized water (or equivalent)

Formulation

Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Format

BSA Free

Preservative

0.01% Sodium Azide

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store lyophilized antibody at 4C. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.

Background: Alkaline Phosphatase, Intestinal

Alkaline phosphatase removes phosphate groups from the 5' end of DNA and RNA, and from proteins, at high pH. Most mammals have 4 different isozymes - placental, placental-like, intestinal and non-tissue specific (found in liver, kidney and bone).

Long Name

Alkaline Phosphatase, Intestinal Type

Alternate Names

alkaline phosphatase, intestinal, Alkaline phosphomonoesterase, ALPI, EC 3.1.3.1, Glycerophosphatase, IAP, Intestinal alkaline phosphatase, intestinal-type alkaline phosphatase, Kasahara isozyme

Gene Symbol

ALPI

UniProt

P19111 (Bovine)

Additional Alkaline Phosphatase, Intestinal Products

Product Documents for Alkaline Phosphatase, Intestinal Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Alkaline Phosphatase, Intestinal Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.