Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-47993
![Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6)Azide and BSA Free [NBP2-47993]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47993-img0002.jpg "Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6)Azide and BSA Free [NBP2-47993]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Flow Cytometry, Immunofluorescence, Immunocytochemistry/ Immunofluorescence, CyTOF-ready
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # PL8-F6
Format
Azide and BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
Purified human Alkaline Phosphatase/ALPP protein (Uniprot: P05187)
Localization
Cytoplasmic and cell surface
Specificity
Reacts with a 70kDa membrane-bound isozyme (Regan and Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP and shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors and can discriminate between these and other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic, and urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.
Marker
Germ Cell Tumor Marker
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Theoretical MW
70 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-44959).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6)Azide and BSA Free [NBP2-47993]
Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] - Western Blot Analysis of JEG-3 cell lysate using PLAP MAb (PL8-F6).![Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993]](https://resources.bio-techne.com/images/products/Alkaline-Phosphatase-ALPP-Antibody-PL8-F6-Azide-and-BSA-Free-Immunohistochemistry-Paraffin-NBP2-47993-img0001.jpg "Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993]")
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993]
Immunohistochemistry-Paraffin: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] - Human Placenta stained with PLAP Monoclonal Antibody (PL8-F6).
Western Blot: Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free [NBP2-47993] -
Validation of EV-CATCHER customized antibodies and characterization of placental EVs. (a). Western blots displaying the specificity of the monoclonal placental alkaline phosphatase antibody against the PLAP recombinant protein (Novus, Centennial, CO, USA, #NBP2-52266) used for EV-CATCHER customization versus the alkaline phosphatase, tissue-nonspecific isozyme antibody against the ALPL recombinant protein (Novus, Centennial, CO, USA, #2909-AP-010). (b). EV-CATCHER assay customized with an anti-PLAP antibody conjugated to a uracilated DNA linker that is connected by a biotin to the bottom of a streptavidin coated well. Using uracil DNA glycosylase (UNG), the immobilized EV-anti-PLAP duplex is released by enzymatic digestion of the double-stranded uracilated DNA linker. (c). Representative Transmission electron microscopy (TEM) images of EVs isolated from placenta previa and placenta percreta maternal plasma, scale bar 100 um. (d). Representative ONi super resolution images of EVs isolated from placenta previa and placenta percreta maternal plasma displaying both high PLAP+ expressing EVs and low PLAP+ expressing EVs, scale bar of 20 um for large field-of-view images and 200 nm for individual representative EVs. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38791142), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
Application
Recommended Usage
Flow Cytometry
0.5 - 1 ug/million cells in 0.1 ml
Immunocytochemistry/ Immunofluorescence
1-2 ug/ml
Immunofluorescence
0.5 - 1.0 ug/ml
Immunohistochemistry-Frozen
0.25 - 0.5 ug/ml
Immunohistochemistry-Paraffin
0.25 - 0.5 ug/ml
Application Notes
Immunohistochemistry (Frozen & Formalin-fixed): 0.25-0.5ug/ml for 30 minutes at RT. No special pretreatment is required for the immunohistochemical staining of formalin-fixed tissues.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: Alkaline Phosphatase/ALPP
Long Name
Alkaline Phosphatase, Placental Type
Alternate Names
Alkaline Phosphatase, PLAP, SEAP
Gene Symbol
ALPP
Additional Alkaline Phosphatase/ALPP Products
Product Documents for Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
Product Specific Notices for Alkaline Phosphatase/ALPP Antibody (PL8-F6) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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