AGER Antibody (2A11) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11993
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Western Blot, ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Surface Plasmon Resonance
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Mouse IgG1 kappa Clone # 2A11
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human AGER protein.
Specificity
2A11 is specific for the V-domain of human advanced glycosylation end product-specific receptor. It recognises both the glycosylated and non-glycosylated forms.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1 kappa
Description
Novus Biologicals Mouse AGER Antibody (2A11) - Azide and BSA Free (NBP3-11993) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Applications for AGER Antibody (2A11) - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Flow Cytometry
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Immunohistochemistry
Optimal dilutions of this antibody should be experimentally determined.
Surface Plasmon Resonance
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
ELISA confirmed the specificity of 2A11 for the V-domain of AGER, with a binding affinity (Kd) of 7.6 nM. ELISA was also used to confirm that the binding of 2A11 to AGER is glycosylation independent. In SPR experiments the Kd of 2A11 binding to AGER was determined as 0.38 nM. Flow cytometry and immunochemistry were used to visualise the binding of 2A11 to HEK-AGER cells.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: RAGE/AGER
Long Name
Receptor for Advanced Glycation End Products
Alternate Names
AGER, SCARJ1
Gene Symbol
AGER
Additional RAGE/AGER Products
Product Documents for AGER Antibody (2A11) - Azide and BSA Free
Product Specific Notices for AGER Antibody (2A11) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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