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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # OTI1D2

Concentration

1 mg/ml

Product Summary for ACADS Antibody (OTI1D2)

Immunogen

Human recombinant protein fragment corresponding to amino acids 34-260 of human ACADS (NP_000008) produced in E.coli.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

41.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for ACADS Antibody (OTI1D2)

Western Blot: ACADS Antibody (OTI1D2) [NBP2-45474] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACADS.
Immunohistochemistry: ACADS Antibody (OTI1D2) [NBP2-45474] - Analysis of Human pancreas tissue.
Western Blot: ACADS Antibody (OTI1D2) [NBP2-45474] - Analysis of extracts (35ug) from 9 different cell lines (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).

Applications for ACADS Antibody (OTI1D2)

Application
Recommended Usage

Immunohistochemistry

1:150

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: ACADS

ACADS encodes a a tetrameric mitochondrial flavoprotein, which is a member of the acyl-CoA dehydrogenase family. This enzyme catalyzes the initial step of the mitochondrial fatty acid beta-oxidation pathway. Mutations in this gene have been associated with Short Chain Acyl-CoA Dehydrogenase Deficiency. [provided by RefSeq]

Alternate Names

ACAD3, acyl-CoA dehydrogenase, C-2 to C-3 short chain, acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain, Butyryl-CoA dehydrogenase, EC 1.3.99, EC 1.3.99.2, mitochondrial short-chain specific acyl-CoA dehydrogenase, SCADshort-chain acyl-CoA dehydrogenase, short-chain specific acyl-CoA dehydrogenase, mitochondrial, unsaturated acyl-CoA reductase

Gene Symbol

ACADS

Additional ACADS Products

Product Documents for ACADS Antibody (OTI1D2)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for ACADS Antibody (OTI1D2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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