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Key Product Details

Species Reactivity

Validated:

Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG3 Clone # 3F7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for 7-Ketocholesterol Antibody (3F7)

Immunogen

Synthetic 7-Ketocholesterol conjugated to Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for 7-ketocholesterol modified proteins.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG3

Scientific Data Images for 7-Ketocholesterol Antibody (3F7)

Western Blot: 7-Ketocholesterol Antibody (3F7) [NBP2-59357] - Western Blot analysis of 7-Ketocholesterol-BSA Conjugate showing detection of 67 kDa 7-Ketocholesterol protein using Mouse Anti-7-Ketocholesterol Monoclonal Antibody, Clone 3F7 (NBP2-59357). Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 ug). Lane 3: BSA (2.0 ug). Lane 4: 7-ketocholesterol-BSA (0.5 ug). Lane 5: 7-ketocholesterol-BSA (2.0 ug). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody (NBP2-59357) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
Immunocytochemistry/Immunofluorescence: 7-Ketocholesterol Antibody (3F7) [NBP2-59357] - Tissue: Embryonic kidney cells (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-7-Ketocholesterol Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). . Courtesy of: Dr. Robert Burke, University of Victoria.

Applications for 7-Ketocholesterol Antibody (3F7)

Application
Recommended Usage

ELISA

1:1000

Immunocytochemistry/ Immunofluorescence

1:50

Western Blot

1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS (pH 7.4), 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Background: 7-Ketocholesterol

Alternate Names

7KC, 7-KC, 7KC-Modified KLH

Additional 7-Ketocholesterol Products

Product Documents for 7-Ketocholesterol Antibody (3F7)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for 7-Ketocholesterol Antibody (3F7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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