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High Throughput Superoxide Dismutase Assay Kit

Bio-Techne includes R&D Systems | Catalog # 7501-500-K

Determines relative SOD activity by measuring the percent inhibition of the rate of formation of WST-1 formazan.

Key Product Details

Features: Analyzes Superoxide Dismutase (SOD) activity in cell extracts using a 96-well plate.
High Throughput Superoxide Dismutase Assay Kit

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FAQs for High Throughput Superoxide Dismutase Assay Kit

  • Can this kit be used to measure serum or plasma samples?

    This kit may be used to measure SOD activity in serum or plasma samples within the detection range of the assay.

  • Can this kit be used to measure saliva samples?

    This kit may be used to measure SOD activity in saliva samples within the detection range of the assay.

  • What is the Lower Limit of Detection (LLD) of this kit?

    An exact LLD has not been established for this kit. However, the lowest amount that is recommended for the standard curve is 0.1 Unit of SOD. The standard curve is based on a purified enzyme.

  • Can frozen tissues be used in this assay?

    We have not tested frozen tissue in this assay. The protocol is based on isolating SOD from fresh tissue. We recommend isolating the cytosolic extracts prior to freezing the samples. Fresh tissue is recommended because single cell suspension from  tissue extracts is needed to eventually prepare cytosolic extracts. It is very difficult to make single cell extracts from frozen tissue and most of the cells have likely already lysed from freezing. 

  • Which form of SOD does this kit detect?

    This kit detects all SOD forms.

Product Documents for High Throughput Superoxide Dismutase Assay Kit

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