Fluorescein-NAD+
Tocris Bioscience, part of Bio-Techne | Catalog # 6574
Fluorescent NAD+; substrate for ADP-ribosylation for use in PARP assays
Discontinued Product
6574 has been discontinued.
View all Fluorescent Enzyme Probes and Enzyme Substrates products.
Key Product Details
Description
Fluorescent NAD+; substrate for ADP-ribosylation for use in PARP assays
Product Description
Fluorescein-NAD+ is a fluoroscein NAD+ is a substrate for ADP-ribosylation, providing a convenient non-isotopic alternative to radiolabelled NAD for use in PARP assays. Allows direct measurement of NAD-dependent enzymes, such as PARP, by fluorescence microscopy. Wavelengths are compatible with use as an acceptor dye in TR-FRET assays, together with the CoraFluor™ 1 (Cat. No. 7920) TR-FRET donor.This product is a replacement for R&D Systems product 4673-500-01
81 μg is supplied as 250 μl of a 0.25 mM solution in water.
Optical Data
| Application | Fluorescence Microscopy |
Product Specifications
Molecular Weight
1291.11
Formula
C56H64N10O22P2
Storage
Store at -80°C
Chemical Name
β-Nicotinamide-N6-[2-[[6-[fluorescein]-amino]-2-oxohexyl-hexyl]amino]-2-oxoethyl]adenine dinucleotide
InChI Key
QHUOJAOGTDUAPK-JDUNSKHUSA-N
SMILES
O=C(CCCCCNC(C1=CC(C(O)=O)=C(C2=C(C=C3)C(OC4=C2C=CC(O)=C4)=CC3=O)C=C1)=O)NCCCCCCNC(CNC5=C(N=CN6[C@@H]7O[C@@H]([C@H]([C@H]7O)O)COP(O)(OP(OC[C@H]8O[C@@H]([N+]9=CC=CC(C(N)=O)=C9)[C@H](O)[C@@H]8O)([O-])=O)=O)C6=NC=N5)=O
The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Solubility
| Solubility | Soluble in water (supplied pre-dissolved at a concentration of 0.25mM) |
Calculators
Molarity Calculator
Dilution Calculator
Reconstitution Calculator
Product Specific Notices for Fluorescein-NAD+
For research use only
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