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BrdU Cell Proliferation Assay Kit (Colorimetric)

Catalog # NBP2-54888 | Novus Biologicals a Bio-Techne Brand
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NBP2-54888

Key Product Details

Sample Type & Volume Required Per Well

Adherent and suspension cells

Product Specifications

Kit Type

Assay Kit (Colorimetric)

Reactivity

Human, Mouse, Rat, Fish, Mammal

Description

5-bromo-2-deoxyuridine (BrdU) is a pyrimidine analog. It gets incorporated into the newly synthesized DNA of proliferating cells in place of thymidine. The BrdU Cell Proliferation Assay Kit detects incorporated BrdU using a mouse anti-BrdU antibody. An anti-mouse HRP-linked secondary antibody is used to detect the anti-BrdU antibody bound to BrdU, which is followed by addition of TMB (a HRP substrate). The extent of color development is proportional to the quantity of BrdU incorporated into the cells and can be used directly as an indicator of cell proliferation. Compared to other cell proliferation assays, this kit detects only the proliferating cells and not the seeded cells. This highly sensitive, non-radioactive kit detects as few as 50-100 proliferating cells.
    Detection method: Absorbance at 450 nmApplications: Detection and quantification of cell proliferation induced by growth factors, cytokines, mitogens, and nutrients. Analysis of cytotoxic and cytostatic compounds such as anticancer drugs, toxic agents and other pharmaceuticals. Determination of the inhibitory or stimulatory effects of various compounds on cell proliferation.


This product is manufactured by Abcam and distributed by Novus Biologicals. (Abcam Catalog Number: ab287841)

Kit Contents for BrdU Cell Proliferation Assay Kit (Colorimetric)

  • Anti-mouse HRP-linked Antibody (2000X)
  • Antibody Diluent
  • BrdU (1000X)
  • BrdU Detection Antibody (300X)
  • Fixing/Denaturing Solution
  • Stop Solution
  • TMB Substrate
  • Wash Buffer (10X)

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C.

Product Documents for BrdU Cell Proliferation Assay Kit (Colorimetric)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for BrdU Cell Proliferation Assay Kit (Colorimetric)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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