APO-BRDU (TUNEL) Apoptosis Kit

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-31161

Novus Biologicals, part of Bio-Techne
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NBP2-31161
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Key Product Details

Applications

Flow Cytometry, Immunocytochemistry/ Immunofluorescence

Kit Type

Apoptosis Kit

Product Summary for APO-BRDU (TUNEL) Apoptosis Kit

The APO-BRDU™ kit is a two color TUNEL assay for labeling DNA breaks (FITC) along with total cellulalr DNA (Propidium Iodide) to detect apoptotic cells. The kit contains all necessary reagents including positive and negative controls.

Product Specifications

Application Notes

Please centrifuge the TdT Enzyme for 30 seconds, upon warming, to ensure all liquid is at the bottom of the tube.

Scientific Data Images for APO-BRDU (TUNEL) Apoptosis Kit

APO-BRDU (TUNEL) Apoptosis Kit [NBP2-31161] - Figure showing the addition of Br-dUTP catalyzed by TdT to the 3'-OH sites of DNA strand breaks induced in the genome of apoptotic cells.
APO-BRDU (TUNEL) Apoptosis Kit [NBP2-31161] - Figure showing apoptotic cells undergoing apoptosis in S-phase.

Kit Contents for APO-BRDU (TUNEL) Apoptosis Kit

  • Br-dUTP
  • Fluorescein-anti-BrdU antibody
  • Negative Control Cells
  • Positive Control Cell
  • Propidium Iodide/RNase DNA staining solution
  • Reaction Buffer
  • Rinsing Buffer
  • TdT Enzyme
  • Wash Buffer

Formulation, Preparation, and Storage

Concentration

Concentration is not relevant for this product. Please see the protocols for proper use of this product.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Storage

Storage of components varies. See protocol for specific instructions.

Background: APO-BRDU (TUNEL)

Apoptosis is the term that describes regulated cell death. It is believed to take place in the majority of animal cells. It is a distinct event that triggers characteristic morphological and biological changes in the cellular life cycle. It is common during embryogenesis, normal tissue and organ involution, cytotoxic immunological reactions and occurs naturally at the end of the life span of differentiated cells. It can also be induced in cells by the application of a number of different agents including physiological activators, heat shock, bacterial toxins, oncogenes, chemotherapeutic drugs, ultraviolet and gamma radiation. When apoptosis occurs, the nucleus and cytoplasm of the cell often fragments into membrane-bound apoptotic bodies that are then phagocytized by neighboring cells. An alternative mode of cell death, necrosis, occurs as a result of gross injury to cells resulting in cellular lysing and release of cytoplasmic components into the surrounding environment, Necrosis often induces an inflammatory response in the tissue. A landmark of cellular self-destruction by apoptosis is the activation of nucleases that degrade the higher order chromatin structure of the DNA into fragments of 50 to 300 kilobases and subsequently into smaller DNA pieces of about 200 base pairs in length. Numerous reviews of the events accompanying apoptosis are available and several well-researched model systems have been described.

Alternate Names

APO BRDU, APO-BRDU, APO-BRDU (TUNEL) Assay, TUNEL Assay

Additional APO-BRDU (TUNEL) Products

Product Documents for APO-BRDU (TUNEL) Apoptosis Kit

Product Datasheets

Certificate of Analysis

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Product Specific Notices for APO-BRDU (TUNEL) Apoptosis Kit

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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