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Featured IHC Products

Microscopic image of tissue sections using immunohistochemistry technique.

Antibodies for Spatial Biology

Enhance your spatial phenotyping experiments with our comprehensive catalog of spatial-ready primary antibodies. Our IHC-validated antibodies are conjugation-ready (BSA and Azide-free) for convenient panel development in multiplexed spatial biology applications.

Microscopic image of tissue sections of human breast cancer detecting Ki-67/MKI67 using Dual RNAscope ISH and IHC technique.

Antibodies for Proteogenomics

Amplify your multiomics research with Bio-Techne utilizing our Dual RNAscope ISH-IHC validated antibodies. By integrating staining strategies from Advanced Cell Diagnostics™, R&D Systems™, and Novus Biologicals™, we enable simultaneous detection of RNA and protein within the same tissue section.

Antibodies Visucyte HRP Illustration

Visu-Cyte HRP Polymer

Uncover the most intricate details within tissue samples with Visu-Cyte HRP Polymer, designed to ensure maximum signal amplification and minimal background noise.

Immunohistochemistry Primary Antibodies

Selecting the right primary antibody is crucial for successful IHC experiments. Consider factors like species reactivity, tissue, subcellular localization, and post translational modifications (PTMs) when selecting a high-quality antibody that specifically binds the target antigen. Bio-Techne offers a robust catalog of highly sensitive antibodies, ensuring target specificity and minimal cross-reactivity. Explore our extensive data image library showcasing the performance of our IHC-validated antibodies across different tissues, applications, and species.

IHC Staining Protocols

IHC can be broadly classified into two forms based on the type of tissue processing involved: IHC- formalin-fixed, paraffin-embedded (FFPE), and IHC-frozen (Fr). Additionally, IHC results can be visualized by either immunofluorescence (IF) or by a chromogenic reaction. The tissue processing type and the intended detection method will inform the IHC staining workflow. Find protocols for each type of tissue and detection method below to expedite your IHC experiments.


Secondary Antibodies and Detection Reagents

Secondary antibodies allow signal amplification and enhance sensitivity in IHC experiments by recognizing and binding to the primary antibodies. Both primary and secondary antibodies are conjugated to detection reagents, such as enzymes or fluorochromes to visualize antigen localization and expression.


IHC Secondary Antibodies by Detection Method

Chromogenic Detection Fluorescent Detection Signal Amplification
HRP Primary Antibodies* Conjugated Primary Antibodies Biotin Conjugated Secondary Antibodies*
HRP Secondary Antibodies* Conjugated Secondary Antibodies HRP Polymer

*Available from Novus Biologicals.


Hela Cells Stain with DAPI image

TSA Vivid™ Fluorophore Kits

Enhance target detection with TSA Vivid dyes, known for their exceptional brightness, especially for low-abundance targets. These dyes significantly improve signal-to-noise ratios in spatial biology applications such as IHC, ICC and ISH. Optimized specifically for the RNAscope™ Multiplex Fluorescent v2 Assay, TSA Vivid dyes ensure reliable and precise results.

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Antibodies for Spatial Biology Reference Table

Looking for antibodies validated for spatial biology? Use our reference table to explore the growing list of Bio-Techne antibodies used in spatial biology, including Nanostring GeoMx Digital Spatial Profiler (DSP), IBEX, and Imaging Mass Cytometry (IMC).

Tissue Samples and Controls

Proper controls are critical to ensuring accurate interpretation of your IHC data. Bio-Techne offers tissue control slides that are important for identifying the source of any staining issues. Other controls like blocking peptides and isotype controls help verify the specificity of antigen-antibody interactions.

Blocking Peptides* Isotype Controls Tissue Control Slides*

*Available from Novus Biologicals.


Support Products for IHC

Bio-Techne offers a variety of support products to optimize your IHC workflows. Our products offer a wide array of benefits like reducing non-specific binding and background noise, vibrant staining, and high-throughput analysis. Explore all support products to enhance specificity, sensitivity, and overall experimental success in IHC.

Antigen Retrieval Buffer* Blocking Serum* DAPI*
MitoBrilliant™ Dyes Tissue Microarrays (TMAs)* Mounting Media*
Tyramide Signaling Amplification (TSA) BSA Janelia Fluor® Dyes

*Available from Novus Biologicals.


Fluorescent Dyes and Probes Brochure

Fluorescence imaging incorporates many types of techniques for visualization of cells and sub-cellular components in live and fixed cells. Techniques include flow cytometry, IHC, ICC, FISH and many more. Our brochure can help you to select the most appropriate dye or probe to obtain the best results for your experiment, providing a background to the use of fluorescent dyes, probes and stains and listing our extensive product range.  

Background Information, Understanding IHC, FAQs

  1. Buchwalow IB, Böcker W (2010) Immunohistochemistry: basics and methods. Springer

  2. Lin F, Prichard J (eds.) (2015) Handbook of practical immunohistochemistry, frequently asked questions. Springer

  3. Kalyuzhny, AE (ed.) (2017) Signal Transduction Immunohistochemistry: Methods and Protocols. Humana Press 1:(Methods in Molecular Biology, 1554)..

  4. Kalyuzhny, AE (ed.) (2022) Signal Transduction Immunohistochemistry: Methods and Protocols. Humana Press 3:(Methods in Molecular Biology, 2593)..

  5. Kalyuzhny, AE (2016) Immunohistochemistry: Essential Elements and Beyond. Springer (Techniques in Life Science and Biomedicine for the Non-Expert)..

  6. Burry, RW (2010) Immunocytochemistry, a practical guide for biomedical research. Humana Press

  7. Oliver C, Jamur MC (eds.) (2010) Immunocytochemical methods and protocols. Humana Press

  8. Mondal PP, Diaspro A (2014) Fundamentals of fluorescence microscopy exploring life with light. Springer, New York

  9. Verveer PJ (ed.) (2015) Advanced fluorescence microscopy, methods, and protocols. Humana Press

  10. Diaspro A (ed.) (2011) Optical fluorescence microscopy, from the spectral to the nano dimension. Springer

  11. Chiarini-Garcia H, Melo R (eds.) (2011) Light microscopy, methods, and protocols. Humana Press

  12. Wiley, Chichester (1993) Immunohistochemistry II. ACCuello (ed.) ISBN-13:9780471934608.

  13. Johnstone AP, Turner MW (eds.) (1997) Immunochemistry: a practical approach. IRL Press, Oxford. 2:ISBN:0199636079.