See Where Your Oligonucleotide Therapeutic Goes and What It Does
Developing effective oligonucleotide therapeutics requires more than confirming that an ASO or siRNA reached the target organ. You need to know which cells received the therapeutic and whether it produced the intended biological effect in those same cells.
Powered by RNAscope™ technology, Bio-Techne Spatial enables spatial, single-cell analysis of therapeutic biodistribution, cell-type tropism, target engagement, and pharmacodynamic response directly in intact tissue.
Stop by the Bio-Techne Spatial table at OTS 2026 and speak with our experts about your RNA therapeutics research.
Go Beyond Bulk Biodistribution
Bulk assays can quantify therapeutic and target RNA levels across a tissue, but they cannot reveal which cells received the therapeutic or whether target modulation occurred specifically within those cells.
RNAscope spatial assays provide the cellular and morphological context needed to connect delivery with biological response.
Map Biodistribution
Directly visualize therapeutic ASOs, siRNAs, and other short RNA sequences in tissue to determine where your therapeutic distributes and persists.
Resolve Cell-Type Tropism
Co-detect your therapeutic with RNA or protein cell-type markers to identify the specific populations reached within heterogeneous tissues.
Measure Target Engagement and Efficacy
Visualize target mRNA alongside your therapeutic to determine whether target modulation occurs within therapeutic-positive cells, rather than relying only on whole-tissue averages.
Evaluate Durability and Safety
Compare therapeutic distribution and target modulation across time points and assess exposure in unintended tissues or cell populations while preserving tissue morphology.
Connect Delivery to Efficacy at Single-Cell Resolution
For an oligonucleotide therapeutic to work, reaching the tissue is only the first step.
RNAscope-based spatial analysis can connect:
Therapeutic delivery → Cell identity → Target modulation → Biological response
This spatial context can help distinguish a delivery problem from a mechanism problem and provide a clearer picture of productive delivery during preclinical development.
Spatial Tools for Oligonucleotide Therapeutic Development
miRNAscope™
Detect short and chemically modified sequences, including ASOs, siRNAs, miRNAs, and shRNAs, directly in tissue to study biodistribution and persistence.
RNAscope™
Detect endogenous target transcripts and pathway markers to assess target engagement and pharmacodynamic response.
BaseScope™
Resolve sequence-specific events including splice variants, exon skipping or inclusion, and edited transcripts.
RNAscope™ Plus + Protein
Combine therapeutic RNA, target mRNA, and cell-type protein markers in the same tissue section to connect tropism with efficacy.