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Webinar: Rapid and Easy Charge Heterogeneity

Webinar Summary

Determination of protein isoelectric points and charge isoforms are critical steps in protein characterization, biopharmaceutical and vaccine development. Charge variants commonly occur as a result of post translational modification including glycosylation, glycation, amidation, sialylation and oxidation. These changes can affect biological activity, patient safety, and drug stability. The iCE3 provides a rapid 10 minute platform method for charge heterogeneity that can be utilized for a wide variety of samples such as purified and recombinant proteins, antibody drug conjugates, vaccines, and viruses. In this webinar Scott Mack, Senior Scientist at ProteinSimple and David Gervais, Senior Process Scientist, Public Health UK will present how iCE can be used for charge heterogeneity analysis of difficult to analyze proteins.

Learning Objectives:

  • How the ProteinSimple iCE system can resolve deamidation, C-terminal lysines, sialylation, oxidation, glycoslyation glycation and any other change to the protein that will result in a change to the pI of the protein
  • How the iCE system can be implemented across the entire pharmaceutical process, from formulation development and optimization, to commercial quality control (QC) release and stability activities
  • How the iCE platform can perform free solution IEF in a capillary column (cIEF) and detect focused protein zones in 10 minutes
  • How this system can be applied across the therapeutic protein development process from discovery through formulation and process development


Scott Mack, Senior R&D Scientist, ProteinSimple

Dave Gervais, Senior Process Scientist, Public Health England