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Webinar: Fast Track Your Phosphorylation Research with Stellar - a NEW Fluorescence Detection Technology

Webinar Summary

Studying protein phosphorylation is a challenging assay using the traditional chemiluminescence Western blotting approach.  Multi-color fluorescence Western blot approaches often fail due to the weaker signal generated by the phospho-specific antibody.  In this webinar, Yan Qian from Dr. Paul Pfaffinger’s lab at Baylor College of Medicine will share her experience in transitioning from traditional chemiluminescence Western blots to automatic immunoassays using ProteinSimple’s Jess™ System with the eIF2a and p-eIF2a (Ser51) detection as an example.  They found the Bio-techne ProteinSimple Jess System combined with the new Stellar fluorescence detection allows simultaneous measurement of phospho and total protein level in just a few hours and the high linearity and reproducibility of the detection module made it easy to create reliable, quantitative measures of changes in eIF2a phosphorylation.

Watch this webinar where you will learn how:

  • Limitation in direct fluorescence detection for several commercially available eIF2a antibodies tested.
  • Switching to the new Stellar fluorescence detection system enabled identification of multiple antibody combination for simultaneous detection of eIF2a and p-eIF2a (Ser51) and a third control in a single run.
  • Linearity and reproducibility of the high-sensitive Stellar fluorescent signal.


Yan Qian, Ph.D.,
Dr. Paul Pfaffinger's Lab
Department of Neuroscience
Baylor College of Medicine