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Webinar: Easy and Simple Charge Heterogeneity Analysis

Webinar Summary

Analyzing charge variants of therapeutic proteins is critical for development and manufacture of a safe, efficacious drug. Types of charge variants include deamidation, formation of N-terminal pyroglutamate, aggregation, isomerization, sialylated glycans, antibody fragmentation, and glycation at the lysine residues. In some cases, such changes affect binding, biological activity, patient safety, and shelf life.

Automated methods for charge heterogeneity detection such as imaged capillary isoelectric focusing (icIEF) have been widely adopted by the biopharma industry. These techniques provide rapid high resolution charge variant separation with easy method development and minimal start up time.

In this webinar we will present a rapid and easy workflow for icIEF method development on the iCE3. The presenters will provide a step-by-step guide for optimizing separations along and review critical parameters for ensuring long term robustness of the assay. Following these simple procedures combined with a 10 minute analysis time allows for robust methods development in an afternoon.



Scott Mack, Senior Scientist, ProteinSimple

Jiaqi Wu, Principal Scientist, ProteinSimple

Susan Darling, Director, Product Marketing, ProteinSimple