Webinar: Combining RNAscope™ and NanoString to study cellular heterogeneity in the lower urinary tract
Anushka Dikshit, Ph.D.
Kit Fuhrman, Ph.D.
Diya Binoy Joseph, Ph.D.
Integrating the capabilities of the ACD, a Bio-Techne brand’s RNAscope in situ hybridization (ISH) probes, for single cell detection of gene expression within the spatial and morphological tissue, with NanoString’s GeoMx Digital Spatial Profiler, for highly sensitive spatial transcriptomics. This webinar will provide a high-level overview of these technologies along with a biological, real world study from UT Southwestern Medical Center.
The lower urinary tract, composed of the bladder and prostate, contains a diverse collection of epithelial and stromal cell types. While single cell RNA-sequencing technology has accelerated the discovery of novel epithelial and stromal cell types in the lower urinary tract, spatial localization, and validation of resolved cell clusters is key to making biological sense of the data. Our lab at UT Southwestern Medical Center in Dallas, has used RNAscope extensively to identify the distributions of spatially distinct fibroblast populations in the human and mouse prostate. Fibroblast distributions in different histologic phenotypes in Benign Prostatic Hyperplasia have also been elucidated by using specific RNAscope probes. RNAscope has completely removed limitations placed by antibody availability. Using RNAscope, it is now extremely easy for us to identify mRNAs corresponding to secreted proteins that would have been impossible to detect with antibodies. We are currently gearing up to use multiplexed RNAscope probes as morphology markers for NanoString GeoMx DSP to detect transcriptomic changes in atrophied glands from prostate hyperplasia patients after 5-alpha reductase inhibitor treatment.