Webinar: AAV Purity Assessment with Capillary Electrophoresis Webinar

Recombinant adeno-associated virus (AAV) is the leading viral vector for the treatment of rare genetic diseases. Like other biologics, AAVs require rigorous manufacturing and testing procedures to ensure vector consistency.

A key challenge within gene therapy manufacturing today is that many analytical tools are required for measuring these critical quality attributes (CQAs). This requires significant capital investment, training, and space, meaning significant resources are used for characterization with fewer available for release testing.

Here we show a single capillary electrophoresis platform (Maurice) capable of multiple purity measurements, including capsid protein ratio and capsid content (empty/full), thereby reducing the number of instruments and methods required for vector characterization. The platform provides stability-indicating methods, meaning Maurice can be used in analytical development and formulation. as well as for quality control (QC) and lot release.

What You'll Discover

• Critical Quality Attributes for Viral Vectors: Explore the essential characteristics that underpin the safety and efficacy of viral vectors in gene therapy
• CE-SDS for Capsid Protein Ratio and Viral Identity: Learn how CE-SDS assesses capsid protein ratio and ensures viral identity
• Imaged cIEF Technique for Capsid Content and Particle Stability: Delve into the capabilities of imaged cIEF in assessing capsid content (empty/full ratios) and ensuring particle stability
• Applications in Analytical Development: Understand how these methods optimize and enhance analytical development, formulation, and QC

Speaker

Chris Heger
Director, Applications Science, Bio-Techne