Validating Targets for Targeted Protein Degradation Using dTAG - A Comprehensive Workflow Solution

This poster summarizes two case studies illustrating the Bio-Techne dTAG workflow from custom TAG knock in cell lines for a protein of interest (POI), to different dTAG Degraders available for treatment and subsequent characterization using an automated western blotting platform, Simple Western™. We present data to demonstrate the value of testing both CRBN- and VHL-recruiting dTAG Degraders for each POI target as well as the use of negative control Degraders. We also show the utility of using a FKBP12 antibody for detecting POI dTAG fusion molecules, allowing for a more simplified procedure when probing multiple targets.