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Detailed Characterization of ERK1 and ERK2 Phosphorylation

Application Notes

Application Notes Summary

Simple Western ERK graph gif

Monophospho- and diphospho-ERK isoforms are not resolved by traditional Western blot analysis, and the sample quantity required is relatively large. Charge-based assays on Simple Western™ can distinguish and quantify unphosphorylated, mono, and dual-phosphorylated isoforms of ERK1 and ERK2, allowing a more accurate determination of ERK activation than Western blots provide.

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