Better Housekeeping: Protein Normalization on Jess

Normalizing target protein abundance to the overall amount of protein present in a sample is a more accurate means than using a housekeeping protein for eliminating technical errors and determining fold-change in protein expression. On Jess™, it’s a simple-to-perform added step in a Simple Western size assay. In this application note, we’ll walk you through the protocol and show you that protein normalization on Jess is indeed superior to the housekeeping protein approach.