Automated Profiling of PROTAC-Induced Cereblon Neosubstrate Degradation Using Simple Western

Here, we present data showing the power of using automated Simple Western™ platforms to screen panels of Degrader and IMiD compounds in order to quantify degradation activity. In this study, we demonstrate the time savings achieved by automating these large screens as well as Simple Western's ability to accurately quantify DC₅₀ and D_max values for specific Degraders and IMiDs.