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Application Brief 1035: Phospho-p38 NanoPro Assay

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The Mitogen Activated Protein Kinase p38 is activated by stress stimuli such as cytokines, ultraviolet irradiation, heat shock, and osmotic shock, and are involved in cell differentiation and apoptosis. We show that Interleukin-1 (IL-1) treatment induces phosphorylation of p38 in fibroblasts. In addition, two independent antibodies recognize peaks at pIs 5.4 and 5.8 for phosphorylated p38.

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