Application Brief 1026: HA Epitope Tag NanoPro Assay
Application Notes
Application Notes Summary
Epitope tags are widely used for afinity purification as well as for highly sensitive detection of recombinant proteins. The Htag consists of a short peptide sequence (MAYPYDVPDYASM). Here, EGFP was expressed with a Htag at both the C- and N-terminal of the protein. As expected from the amino acid composition of this tag, a slight acidic shift in the pI of the tagged protein can be observed.
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