Calculate the dilution required to prepare a stock solution
The Bio-Techne dilution calculator enables you to calculate how to dilute a stock solution of known concentration. Enter C1, C2 & V2 to calculate V1.
The dilution calculator equation
The Bio-Techne dilution calculator is based on the following equation:
Concentration (start) x Volume (start) = Concentration (final) x Volume (final)
The dilution equation is commonly abbreviated to: C1V1 = C2V2
An example of a dilution calculation using the Bio-Techne dilution calculator
What volume of a given 10 mM stock solution is required to make 20 ml of a 50 μM solution?
Using the equation C1V1 = C2V2, where C1=10 mM, C2=50 μM, V2=20 mL and V1 is the unknown:
- Enter 10 into the Concentration (start) box and select the correct unit (millimolar)
- Enter 50 into the Concentration (final) box and select the correct unit (micromolar)
- Enter 20 into the Volume (final) box and select the correct unit (milliliter)
- Press calculate
- The answer of 100 microliter (0.1 mL) appears in the Volume (start) box
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