Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73680
Discontinued Product
NBP1-73680 has been discontinued.
View all IgA Heavy Chain products.
Key Product Details
Species Reactivity
Rat
Applications
Flow Cytometry, Fluorophore-linked immunosorbent assay, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Texas Red (Excitation = 589 nm, Emission = 615 nm)
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Rat IgA alpha heavy chain
Specificity
Specificity was confirmed by ELISA at less than 1% against other Rat heavy or light chain combinations.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rat IgA and Rat Serum
Scientific Data Images for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red]
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red] [NBP1-73680] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: None. Secondary antibody: Texas Red goat secondary antibody at 1:1,000 for 60 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): Rat IgA light chain.
Applications for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red]
Application
Recommended Usage
Flow Cytometry
1:500 - 1:2500
Fluorophore-linked immunosorbent assay
1:10000 - 1:50000
Immunocytochemistry/ Immunofluorescence
1:1000 - 1:5000
Application Notes
This product has been tested by western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgA Heavy Chain
Additional IgA Heavy Chain Products
Product Documents for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red]
Product Specific Notices for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Texas Red]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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