Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-73678
Discontinued Product
NBP1-73678 has been discontinued.
View all IgA Heavy Chain products.
Key Product Details
Species Reactivity
Rat
Applications
ELISA, Immunohistochemistry, Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
Concentration
LYOPH mg/ml
Product Specifications
Immunogen
Rat IgA alpha heavy chain
Specificity
No reaction was observed against other Rat heavy or light chain proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Description
Store vial at 4C prior to restoration. For extended storage aliquot contents and freeze at -20C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgA coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgA and Rat Serum
Scientific Data Images for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
Western Blot: Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin] [NBP1-73678] - Lane 1: Mouse IgA Kappa. Lane 2: None. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: incubated with blocking buffer for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None.
![Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]](https://resources.bio-techne.com/images/products/nbp1-73678_goat-polyclonal-goat-anti-rat-iga-heavy-chain-secondary-antibody-biotin-255202313135139.jpg "Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]")
Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
Western Blot of Biotin Conjugated Goat Anti-Rat IgA (Alpha chain) Secondary Antibody. Lane 1: Molecular Weight Marker. Lane 2: Mouse IgA Kappa. Load: 100 ng per lane. Primary antibody: Rat IgA (Alpha chain) antibody biotin conjugated at 1:1,000 for 60 min at RT. Secondary antibody: Peroxidase streptavidin secondary antibody at 1:40,000 for 30 min at RT. Blocking: for 30 min at RT. Predicted/Observed size: 55 kDa, 55 kDa for Rat IgA. Other band(s): None.Applications for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
Application
Recommended Usage
ELISA
1:20000 - 1:100000
Immunohistochemistry
1:1000 - 1:5000
Western Blot
1:2000 - 1:10000
Application Notes
This product has been tested by western blot and assayed against 1.0 ug of Rat IgA in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:10,000 to 1:30,000 of the reconstitution concentration is suggested for this product.
Formulation, Preparation, and Storage
Purification
Multi-step
Reconstitution
Reconstitute with 1.0 ml deionized water (or equivalent).
Formulation
Lyophilized from 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store lyophilized antibody at 4C in the dark. Aliquot reconstituted liquid and store at -20C. Avoid freeze-thaw cycles.
Background: IgA Heavy Chain
Additional IgA Heavy Chain Products
Product Documents for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
Product Specific Notices for Goat anti-Rat IgA Heavy Chain Secondary Antibody [Biotin]
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.
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