Mitochondrial studies in live neurons
Assay Type: In Vitro
Cell Line/Tissue: spinal cord neurons
TMRM fluorescence intensity before and after CCCP addition was used for the quantitation of change in mitochondrial membrane potential in spinal cord neurons induced by glutamate receptor agonist AMPA
Time lapse images of TMRM fluorescence of mitochondria rich regions in neurons was recorded. The fluorescence intensity (F) at a given time point was normalized to the initial fluorescence intensity (F0) before addition of the AMPA. At the end of each experiment CCCP (750 nM) was added to the bath. CCCP collapses the mitochondrial membrane potential and decreases the TMRM fluorescence.