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Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-43527

Novus Biologicals, part of Bio-Techne
Discontinued Product
NBP1-43527 has been discontinued. View all IgM Heavy Chain products.

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Biotin

Antibody Source

Monoclonal Rat IgG2a Kappa

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

Mouse IgM

Reactivity Notes

This antibody is reactive to Mouse.

Specificity

The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2a Kappa

Scientific Data Images for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

Flow Cytometry: Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin] [NBP1-43527] - Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) FITC and 0.25 ug of Rat IgG2a Isotype Control Biotin (left) or 0.25 ug of Anti-Mouse IgM Biotin (right) followed by Streptavidin PE. Total viable cells were used for analysis.

Applications for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) with 0.1% gelatin

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: IgM Heavy Chain

Alternate Names

Constant region of heavy chain of IgM, Ig Mu Chain C Region BOT, Ig Mu Chain C Region GAL, Ig Mu Chain C Region OU, immunoglobulin heavy constant mu, Immunoglobulin mu chain

Gene Symbol

IGHM

Additional IgM Heavy Chain Products

Product Documents for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Rat anti-Mouse IgM Heavy Chain Secondary Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Secondary Antibodies are guaranteed for 1 year from date of receipt.

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