Recombinant Mouse PDGF-CC Protein
Catalog # 1447-PC | R&D Systems, Inc. a Bio-Techne Brand
Key Product Details
Accession #
Source
Structure / Form
Conjugate
Applications
Product Specifications
Source
Val235-Gly345, with an N-terminal Met and a 6-His tag
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 70-350 ng/mL.
Formulation, Preparation and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 1447-PC
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA with BSA as a carrier protein. |
Reconstitution | Reconstitute at 10 μg/mL in sterile 4 mM HCl containing at least 0.1% human or bovine serum albumin. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Carrier Free: 1447-PC/CF
Formulation | Lyophilized from a 0.2 μm filtered solution in Acetonitrile and TFA. |
Reconstitution | Reconstitute at 100 μg/mL in sterile 4 mM HCl. |
Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: PDGF-CC
The platelet-derived growth factor (PDGF) family consists of proteins derived from four genes (PDGF-A, -B, -C, and -D) that form four disulfide-linked homodimers (PDGF-AA, -BB, -CC, and -DD) and one heterodimer (PDGF-AB) (1). These proteins regulate diverse cellular functions by binding to and inducing the homo- or hetero-dimerization of two receptor tyrosine kinases (PDGF R alpha and R beta). Within the PDGF family, PDGF-C and PDGF-D constitute a subgroup that shares similar structural organization (2, 3). Both proteins are secreted as inactive homodimeric latent growth factors. Each monomer has two distinct protein domains: an N-terminal CUB domain; and a C-terminal PDGF/VEGF homology domain that shares 27 - 35% sequence identity with the corresponding regions of other PDGF family members. An 80 - 90 amino acid residue hinge region connects the two domains. Sequential removal of the CUB domains in the homodimeric latent growth factor by extracellular proteolytic cleavage at the hinge region is required to release the bioactive PDGF/VEGF homology domain (1). Twelve cysteine residues are found within the PDGF/VEGF homology domain of PDGF-C, including the characteristic eight invariant cysteine residues involved in inter- and intra-chains disulfide-bonds needed for the formation of the cysteine-knot structure. Bioactive PDGF-CC binds with high-affinity to PDGF R alpha but not PDGF R beta and activates PDGF R alpha homodimerization (1). PDGF-CC has also been shown to activate PDGF R alpha beta heterodimers (1). PDGF-CC is expressed in multiple embryonic and adult cell types and tissues. During embryonic development, PDGF-CC is involved in ductal morphogenesis (4). PDGF-CC is a potent angiogenic factor that stimulates vessel growth in the mouse cornea pocket assay and in the CAM assay (5). It stimulates coronary artery smooth muscle cell proliferation and may play an important role in cardiovascular development and function (6). PDGF-CC is also expressed in many tumors and tumor cell lines and has a causative role in tumorigenesis (7). Mature human and mouse PDGF-C share 93.7% amino acid sequence identity.
References
- Li, X. and U. Eriksson (2003) Cytokine & Growth Factor Rev. 14:91.
- LaRochells, W.J. et al. (2001) Nature Cell Biol. 3:517.
- Li, X. et al. (2000) Nature Cell Biol. 2:302.
- Aase, K. et al. (2002) Mech. Dev. 110:187.
- Cao, R.H. et al. (2002) FASEB J. 16:1575.
- Gilbertson, D. et al. (2001) J. Biol. Chem. 276:27406.
- Zwerner, J.P. and W.A. May (2001) Oncogene 20:626.
Long Name
Alternate Names
Gene Symbol
UniProt
Product Documents for Recombinant Mouse PDGF-CC Protein
Product Specific Notices for Recombinant Mouse PDGF-CC Protein
For research use only
Citations for Recombinant Mouse PDGF-CC Protein (7)
Citations are publications that use Bio-Techne products. Selected citations for Recombinant Mouse PDGF-CC Protein include:
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Species: Mouse
Sample Types: Whole Cells
Applications: BioassayY Geng et al. (2022-11-19), PEAR1 regulates expansion of activated fibroblasts and deposition of extracellular matrix in pulmonary fibrosis Nature Communications, 2022-113(1):7114.
PMID: 36402779 -
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay(2016-08-05), Endothelial PDGF-CC regulates angiogenesis-dependent thermogenesis in beige fat Nat Commun, 2016-07(0):12152.
PMID: 27492130 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayHayes B et al. (2014-03-25), Activation of platelet-derived growth factor receptor alpha contributes to liver fibrosis. PLoS ONE, 2014-09(3):e92925.
PMID: 24667490 -
Species: Mouse
Sample Types: In Vivo
Applications: In VivoJackman K et al. (2013-12-11), Progranulin deficiency promotes post-ischemic blood-brain barrier disruption. J Neurosci, 2013-133(50):19579-89.
PMID: 24336722 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayYang et al. (2013-12-02), Nonneuronal control of the differential distribution of myelin along retinal ganglion cell axons in the mouse. Invest Ophthalmol Vis Sci, 2013-154(13):7819-27.
PMID: 24222305 -
Species: Mouse
Sample Types: Whole Cells
Applications: BioassayMorrison C et al. (2011-07-22), Microarray and Proteomic Analysis of Breast Cancer Cell and Osteoblast Co-cultures: ROLE OF OSTEOBLAST MATRIX METALLOPROTEINASE (MMP)-13 IN BONE METASTASIS. J. Biol. Chem., 2011-0286(39):34271-85.
PMID: 21784845 -
Species: Mouse
Sample Types: N/A,Whole Cells
Applications: Western Blot,BioassayCrawford Y et al. (2009-01-06), PDGF-C mediates the angiogenic and tumorigenic properties of fibroblasts associated with tumors refractory to anti-VEGF treatment. Cancer Cell, 2009-015(1):21-34.
PMID: 19111878
There are no citations that match your criteria.
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