Recombinant Mammalian Ubiquitin, CF

Name: Recombinant Mammalian Ubiquitin, CF
Brand: R&D Systems
SKU: U-100

R&D Systems, part of Bio-Techne | Catalog # U-100

Submit a review

Product Datasheet / COA / SDS

Discontinued Product

U-100 has been discontinued. View all Ubiquitin products.

Key Product Details

Source:	E. coli
Applications:	Enzyme Activity

View Full Technical Details for Recombinant Mammalian Ubiquitin, CF

Technical Data
Citations / Reviews

Product Specifications for Recombinant Mammalian Ubiquitin, CF

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

U-100

Stability & Storage:

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Ubiquitin

Highly purified ubiquitin processed for the quantitative removal of glycine and buffer salts whichcan interfere with chemical and in vitro reactions. Ubiquitin is a 76 amino acid, highly conserved nuclear and cytoplasmic protein. It is found exclusively in eukaryotes, becomes covalentlyattached to substrate proteins by enzymes in the Ubiquitin-Proteosome Pathway (UPP) and has amajor role in targeting cellular proteins for the ATP-dependent degradation by the 26S proteosome. Ubiquitination also affects proteosome-independent events such as protein localization, activity and function.

Item	Value
References	Ciechanover A. et al. (1980) J. Biol. Chem. 255:7525-7528 Coux O. et al. (1996) Ann. Rev. Biochem. 65:801-847 Glickman M. H. and Ciechanover A. (2002) Physiol. Rev. 82:373-428 Hershko A. and Ciechanover A. (1992) Ann. Rev. Biochem. 61:761-807 Schwartz A. L and Ciechanover A. (1999) Ann. Rev. Med. 50:57-74. Wilkinson K. D. and Audhya T. K. (1981) J. Biol. Chem. 256:9235-9241
Entrez Gene IDs	7314 (Human); 298693 (Rat)
Alternate Names	RPS27A, UBA52, UBB, UBB ubiquitin B, UBC, Ubiquitin

Customer Reviews for Recombinant Mammalian Ubiquitin, CF

There are currently no reviews for this product. Be the first to review Recombinant Mammalian Ubiquitin, CF and earn rewards!

Have you used Recombinant Mammalian Ubiquitin, CF?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review

Recombinant Mammalian Ubiquitin, CF

R&D Systems, part of Bio-Techne | Catalog # U-100

Key Product Details

Product Specifications for Recombinant Mammalian Ubiquitin, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Ubiquitin

Customer Reviews for Recombinant Mammalian Ubiquitin, CF

Product Documents for Recombinant Mammalian Ubiquitin, CF

Product Datasheets

COA

Certificate of Analysis

SDS

CONTACT INFORMATION

Stay Connected