Does Recombinant Human Tau 383 (0N4R) Protein, CF (Catalog # SP-499) contain a Caspase-3 cleaved form?

Name: Recombinant Human Tau 383 (0N4R) Protein, CF
Brand: R&D Systems
SKU: SP-499

The Tau protein can get cleaved by the enzyme Caspase-3 after the amino acid residues DEVD or DMVD. The Tau 383 isoform does contain that sequence of residues ending at aa363. However, SP-499 is raised in E. coli, which does not naturally express Caspase-3. Therefore, we would not expect the cleaved form of Tau 383 to be present in SP-499.

Recombinant Human Tau 383 (0N4R) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # SP-499

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Discontinued Product

SP-499 has been discontinued. View all Tau products.

Key Product Details

Source:	E. coli
Accession #:	NP_058518.1
Applications:	Bioactivity

View Full Technical Details for Recombinant Human Tau 383 (0N4R) Protein, CF

Technical Data
Citations / Reviews / FAQs

Product Specifications for Recombinant Human Tau 383 (0N4R) Protein, CF

Source	E. coli-derived human Tau protein Met1 - Leu383
Purity	>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass	40 kDa
SDS-PAGE	40 kDa
Activity	Concentrations for in vitro assays will depend on experimental conditions and detection methods.

Formulation, Preparation and Storage

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

SP-499

Formulation	Supplied as a solution in PBS.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -70 °C as supplied. 3 months, -70 °C under sterile conditions after opening.

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Tau

Tau is a microtubule-associated protein expressed primarily in neurons. Carboxy-terminal domains of Tau associate with and stabilize microtubule structure, while other domains bind to the plasma membrane. Abnormal Tau phosphorylation may result in the self-assembly of tangles of paired helical and/or straight filaments, which are involved in the pathogenesis of Alzheimer's disease and other neurodegenerative diseases. Properly folded Tau is highly soluble, but when the protein becomes misfolded it forms insoluble aggregates that can damage cytoplasmic functions, interfere with axonal transport and ultimately lead to cell death. There are multiple forms of Tau. The product is a 383 amino acid isoform known as "0N4R," "Isoform Tau-D" or "Tau 383" and is referenced in UniProt as P10636-6.

Item	Value
References	Billingsley M.L. & Kincaid R.L. (1997) Biochem. J. 323:577. Bloom G.S. (2014) JAMA Neurol. 71:505. Cripps D. et al. (2006) J. Biol. Chem. 281:10825. Harada A. et al. (1994) Nature 369:488. Lei P. et al. (2010) Int. J. Biochem. Cell Biol. 42:775.
Long Name	Microtubule-Associated Protein Tau
Entrez Gene IDs	4137 (Human); 17762 (Mouse); 29477 (Rat)
Alternate Names	DDPAC, FLJ31424, FTDP-17, G protein beta1/gamma2 subunit-interacting factor 1, MAPT, MGC138549, MSTD, MSTDMAPTL, MTBT1, MTBT1Neurofibrillary tangle protein, MTBT2, Neurofibrillary tangle protein, PHF-tau, PPND, TAUPaired helical filament-tau, Tau, microtubule-associated protein tau

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Recombinant Human Tau 383 (0N4R) Protein, CF

R&D Systems, part of Bio-Techne | Catalog # SP-499

Key Product Details

Product Specifications for Recombinant Human Tau 383 (0N4R) Protein, CF

Formulation, Preparation and Storage

Carrier Free

Reconstitution Calculator

Background: Tau

Customer Reviews for Recombinant Human Tau 383 (0N4R) Protein, CF

FAQs for Recombinant Human Tau 383 (0N4R) Protein, CF

Product Documents for Recombinant Human Tau 383 (0N4R) Protein, CF

Product Datasheets

COA

Certificate of Analysis

SDS

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