Recombinant Human Serpin A7/TBG Protein, CF

R&D Systems, part of Bio-Techne | Catalog # 9564-PI

R&D Systems, part of Bio-Techne
Discontinued Product
9564-PI has been discontinued. View all Serpin A7/TBG products.

Key Product Details

Source

CHO

Accession #

P05543

Conjugate

Unconjugated

Applications

Bioactivity

View all Serpin A7/TBG Proteins and Enzymes »

Product Specifications

Source

Chinese Hamster Ovary cell line, CHO-derived human Serpin A7/TBG protein
Ala21-Ala415, with a C-terminal 6-His tag

Purity

>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.

Endotoxin Level

<0.10 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Ala21

Predicted Molecular Mass

45 kDa

SDS-PAGE

58-66 kDa, reducing conditions

Activity

Measured by its binding ability in a functional ELISA.
When Thyroxine-BSA Conjugate is immobilized at 2.5 μg/mL, 100 μL/well, Recombinant Human Serpin A7/TBG binds with an ED50 of 0.4-2 μg/mL.

Scientific Data Images for Recombinant Human Serpin A7/TBG Protein, CF

Recombinant Human Serpin A7/TBG Protein Bioactivity

Recombinant Human Serpin A7/TBG Protein Bioactivity

When Thyroxine-BSA Conjugate is immobilized at 2.5 μg/mL, 100 μL/well, Recombinant Human Serpin A7/TBG (Catalog # 9564-PI) binds with an ED50 of 0.4-2 μg/mL.

Formulation, Preparation and Storage

9564-PI
Formulation Lyophilized from a 0.2 μm filtered solution in Tris and NaCl.
Reconstitution Reconstitute at 1 mg/mL in water.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A7/TBG

Thyroxine-binding globulin (TBG), also known as Serpin A7, is an approximately 44 kDa member of the serpin superfamily of serine protease inhibitors. TBG belongs to the clade A subgroup of serpins which include inflammatory response molecules (1). Human TBG shares 76% sequence identity to mouse TBG. TBG is one of two serpins known to bind and transport hormones. TBG is responsible for binding the majority of thyroxine (T4) in the blood and releasing it through conformational changes in the reactive loop (2). In addition, cleavage of TBG's reactive site loop by proteases present at inflammatory sites causes a targeted and immediate release of thyroxine from TBG (3). TBG levels are modified by estrogen levels (4-6) and may directly predict perinatal syndromal depression in late pregnancy (6). In addition, elevated TBG levels were a reported marker for chronic obstructive pulmonary disease (7) in support of reported endocrine system dysfunction in patients with the disease (8, 9).

References

  1. Law, R. et al. (2006) Genome Biol. 7:216.
  2. Zhou, A. et al. (2006) Proc. Natl. Acad. Sci. USA 103:13321.
  3. Jirasakuldech, B. et al. (2000) J. Clin. Endocrinol. Metab. 85:3996.
  4. Glinoer, D. (1997) Endocr. Rev. 18:404.
  5. Gaberscek, S. and K. Zaletel (2011) Expert Rev. Clin. Immunol. 7:697.
  6. Pedersen, C. et al. (2016) Psychoneuroendocrinology 65:84.
  7. Diao, W. et al. (2017) Int. J. Chron. Obstruct. Pulmon. Dis. 12: 1549.
  8. Terzano, C et al. (2014) Lung 192:103.
  9. Sarinc Ulasli, S. et al. (2013) Multidiscip. Respir. Med. 8:64.

Alternate Names

TBG

Entrez Gene IDs

6906 (Human); 331535 (Mouse); 81806 (Rat)

Gene Symbol

SERPINA7

UniProt

P05543

Additional Serpin A7/TBG Products

Product Documents for Recombinant Human Serpin A7/TBG Protein, CF

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Certificate of Analysis

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Product Specific Notices for Recombinant Human Serpin A7/TBG Protein, CF

For research use only

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