Recombinant Human RNF4 Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E3-210
Discontinued Product
E3-210 has been discontinued.
View all RNF4 products.
Key Product Details
Product Specifications
Source
E. coli-derived human RNF4 protein
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
22 kDa
Activity
Recombinant Human RNF4 is a Ubiquitin ligase (E3) that functions downstream of a Ubiquitin-activating (E1) enzyme and a Ubiquitin-conjugating (E2) enzyme to conjugate Ubiquitin to substrate proteins. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human RNF4 concentration of 100-500 nM.
Formulation, Preparation and Storage
E3-210
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 200 mM NaCl, 5 mM DTT |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
|
Background: RNF4
RNF4 (small nuclear ring finger protein, SNURF) is a RING-finger ubiquitin E3 ligase that ubiquitinates and mediates the proteasomal destruction of targets such as PML, PEA3, CENP1, and PARP1. In addition to the RING domain, RNF4 contains four SUMO-interacting motifs (SIMs) that function to recruit this ligase to poly-sumoylated substrates. RNF4 will autoubiquitinate in vitro, and will also ubiquitinate poly-SUMO chains.
References
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Geoffroy, M-C et al. (2010) Mol. Bio. Cell 21: 4227
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Tathum, M.H. et al. (2008) Nat. Cell Bio. 10: 538
Long Name
Ring Finger Protein 4
Alternate Names
RES4-26, SLX5, SNURF
Entrez Gene IDs
6047 (Human)
Gene Symbol
RNF4
UniProt
Additional RNF4 Products
Product Documents for Recombinant Human RNF4 Protein, CF
Product Specific Notices for Recombinant Human RNF4 Protein, CF
For research use only
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