Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF
R&D Systems, part of Bio-Techne | Catalog # E-563
Discontinued Product
E-563 has been discontinued.
View all OTUD7B/Cezanne products.
Key Product Details
Product Specifications
Source
E. coli-derived human OTUD7B/Cezanne protein
Asn124 - Leu436
Asn124 - Leu436
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
36 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human Cezanne Catalytic Domain concentration of 0.1-1.0 μM.
Formulation, Preparation and Storage
E-563
| Formulation | 1.8 mg/ml (50 μM) in 50 mM HEPES pH 7.5, 100 mM NaCl, 10 % Glycerol (v/v), 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: OTUD7B/Cezanne
OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappa B (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa. The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked polyubiquitin chains, but this enzyme displays a very strong preference for K11-linked chains in vitro. It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3. This untagged recombinant protein contains the catalytic domain (including the OTU domain) of Cezanne.
References
- Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939
- Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036
- Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180
- Hu, H. et al. (2013) Nature 494: 371
Long Name
OTU Domain-containing Protein 7B
Alternate Names
Cezanne, ZA20D1
Gene Symbol
OTUD7B
UniProt
Additional OTUD7B/Cezanne Products
Product Documents for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF
Product Specific Notices for Recombinant Human OTUD7B/Cezanne Catalytic Domain, CF
For research use only
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