Recombinant Human His6-OTUD7B/Cezanne Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-562
Discontinued Product
E-562 has been discontinued.
View all OTUD7B/Cezanne products.
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human OTUD7B/Cezanne protein
Contains a C-terminal 6-His tag
Contains a C-terminal 6-His tag
Purity
>85%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
93 kDa
Activity
Recombinant Human OTUD7B/Cezanne is a Ubiquitin-specific deconjugating enzyme. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human OTUD7B/Cezanne concentration of 0.1-1 μM.
Formulation, Preparation and Storage
E-562
| Formulation | X mg/ml (X μM) in 50 mM Hepes pH 7.5, 100 mM NaCl, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: OTUD7B/Cezanne
OTU domain-containing protein 7B (OTUD7B), also known as cellular zinc finger anti-NF-kappaB (Cezanne), is a C64 type peptidase and a member of the ovarian tumor (OTU) protein super-family with a predicted molecular weight of 93 kDa (1). The human protein shares 90% amino acid sequence identity with its mouse ortholog. Cezanne has been reported to cleave K48-, and K63-linked poly-Ubiquitin chains, and also to display a preference for K11-linked chains (1-3). It has been shown to play a role in the negative regulation of NF-kappa B signaling, possibly by deubiquitination of RIPK1 and TRAF3 (2,4).
References
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Evans, P.C. et al. (2003) J. Biol. Chem. 278: 23180.
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Enesa, K. et al. (2008) J. Biol. Chem. 283: 7036.
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Bremm, A. et al. (2010) Nat. Struct. Mol. Biol. 17: 939.
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Hu, H. et al. (2013) Nature 494:371.
Long Name
OTU Domain-containing Protein 7B
Alternate Names
Cezanne, ZA20D1
Gene Symbol
OTUD7B
UniProt
Additional OTUD7B/Cezanne Products
Product Documents for Recombinant Human His6-OTUD7B/Cezanne Protein, CF
Product Specific Notices for Recombinant Human His6-OTUD7B/Cezanne Protein, CF
For research use only
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