Recombinant Human GST-AMSH-LP Protein, CF
R&D Systems, part of Bio-Techne | Catalog # E-551
Discontinued Product
E-551 has been discontinued.
View all AMSH-LP products.
Key Product Details
Product Specifications
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived human AMSH-LP protein
Contains an N-terminal GST (glutathione S-transferase) tag
Contains an N-terminal GST (glutathione S-transferase) tag
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain.
Predicted Molecular Mass
79 kDa
Activity
Reaction conditions will need to be optimized for each specific application. We recommend an initial human recombinant GST-AMSH-LP concentration of 100-500 nM when using K63-linked poly-Ubiquitin substrates. AMSH-LP does not efficiently utilize Ubiquitin-AMC (U-550) as a substrate, though a signal may be obtained when the enzyme is used at a concentration of 500 nM or higher.
Formulation, Preparation and Storage
E-551
| Formulation | X mg/ml (X μM) in 50 mM HEPES pH 8.0, 150 mM NaCl, 10% (v/v) Glycerol, 1 mM TCEP |
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage | Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
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Background: AMSH-LP
AMSH-like protease (AMSH-LP, also known as STAM-Binding Protein-Like 1, STAMBPL1) is a zinc metalloprotease belonging to the JAMM (JAB1/MPN/Mov34) family of deubiquitinating enzymes (DUBs). 436 amino acids in length, AMSH-LP is 56% identical and 73% similar to AMSH, an enzyme that functions at the endosome, where it is involved in the sorting of various cell-surface receptors to lysozomes. Like AMSH, AMSH-LP only cleaves K63-linked poly-Ubiquitin chains in vitro; however, unlike AMSH, AMSH-LP does not appear to associate with STAM protein. This recombinant protein is full-length and contains an N-terminal GST tag.
References
- Davies, C.W. et al. (2011) J. Mol. Biol. 413: 416
- Sato, Y. et al. (2008) Nature 455: 358
Long Name
STAM-binding Protein-like 1
Alternate Names
ALMalpha, AMSH-FP, AMSHLP, STAMBPL1
Gene Symbol
STAMBPL1
UniProt
Additional AMSH-LP Products
Product Documents for Recombinant Human GST-AMSH-LP Protein, CF
Product Specific Notices for Recombinant Human GST-AMSH-LP Protein, CF
For research use only
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